CAJ | 학술논문

背景与目的人长链非编码RNA基因的异常表达与多种肿瘤有关。本研究旨在探讨人长链非编码RNA基因SPRY4-IT1对肺癌细胞A549侵袭和迁移能力的影响及机制。方法将SPRY4-IT1转染入肺癌A549细胞系，采用RT-PCR方法检测重组质粒在细胞中表达水平比较；采用MTT和Transwell检测A549细胞侵袭和迁移能力的变化，采用Western blot方法检测SPRY4-IT1对MMP-2和MMP-9蛋白的影响。结果转染pcDNA3.1-SPRY4-IT1细胞划痕两侧细胞间距较转染pcDNA3.1细胞明显变窄，其穿膜细胞数为（207±34）个/视野，相比对照组明显增多（P<0.05），且转染pcDNA3.1-SPRY4-IT1细胞中的基质金属蛋白酶（matrix metalloproteinase, MMP）-2及MMP-9蛋白表达较转染空质粒组均有升高。结论在肺癌A549细胞中过表达SPRY4-IT1可增强细胞的侵袭、迁移能力，且细胞内MMP-2及MMP-9表达升高，提示SPRY4-IT1可能通过调控MMP影响肺癌的侵袭和转移能力。
배경여목적인장련비편마RNA기인적이상표체여다충종류유관。본연구지재탐토인장련비편마RNA기인SPRY4-IT1대폐암세포A549침습화천이능력적영향급궤제。방법장SPRY4-IT1전염입폐암A549세포계，채용RT-PCR방법검측중조질립재세포중표체수평비교；채용MTT화Transwell검측A549세포침습화천이능력적변화，채용Western blot방법검측SPRY4-IT1대MMP-2화MMP-9단백적영향。결과전염pcDNA3.1-SPRY4-IT1세포화흔량측세포간거교전염pcDNA3.1세포명현변착，기천막세포수위（207±34）개/시야，상비대조조명현증다（P<0.05），차전염pcDNA3.1-SPRY4-IT1세포중적기질금속단백매（matrix metalloproteinase, MMP）-2급MMP-9단백표체교전염공질립조균유승고。결론재폐암A549세포중과표체SPRY4-IT1가증강세포적침습、천이능력，차세포내MMP-2급MMP-9표체승고，제시SPRY4-IT1가능통과조공MMP영향폐암적침습화전이능력。
Background and objective hTe abnormal expression of human long chain non encoding RNA gene is related to many kinds of tumors. hTe aim of this study is to investigate the expression of long non-coding RNA maternally expressed gene 3 (SPRY4-IT1) in lung cancer (A549) cells, and to observe the effect of SPRY4-IT1 on the invasion and migra-tion of A549 cells.Methods hTe levels of SPRY4-IT1 in A549 was detected by Real-time PCR. hTe effects of SPRY4-IT1 on the invasion and migration of A549 cell were analyzed by MTT and Transwell assay. hTe expression of matrix metalloproteinase (MMP) family proteins was determined by Western blot.Results hTe invasion and migration of A549 cells were increased atfer SPRY4-IT1 over-expression. hTe cell spaces were narrower atfer SPRY4-IT1 over expression in the wound healing assay. Tran-swell assays showed that the numbers of transmembrane A549 cells were signiifcantly higher in SPRY4-IT1 over expression group than that in control group (P<0.05). Meanwhile, over expression of SPRY4-IT1 reduced the expression of MMP-2 and MMP-9.Conclusion Over expression of SPRY4-IT1 enhanced the invasion and migration of A549 cells. MMP-2 and MMP-9 might play an important role in this regulation.