中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2015年
15期
2864-2869
,共6页
侯巍巍%侯刚%李宁%庞宇
侯巍巍%侯剛%李寧%龐宇
후외외%후강%리저%방우
乳腺肿瘤%蛋白质组学%光谱法,质量,基质辅助激光解吸电离%热休克蛋白质类
乳腺腫瘤%蛋白質組學%光譜法,質量,基質輔助激光解吸電離%熱休剋蛋白質類
유선종류%단백질조학%광보법,질량,기질보조격광해흡전리%열휴극단백질류
Breast neoplasms%Proteomics%Spectrometry,mass,matrix-assisted laser desorption-ionization%Heat-shock proteins
目的：筛选乳腺癌组织与癌旁正常乳腺组织之间的差异蛋白，寻找和鉴定与乳腺癌相关的蛋白质。方法选取新鲜乳腺浸润性导管癌组织及癌旁正常乳腺组织行双向凝胶电泳，得到的差异蛋白点进行图像分析，运用质谱分析对所筛选的差异蛋白点做质谱鉴定，根据检索软件Mascot计算得到蛋白的分值，输入NCBI数据库来获得蛋白质的信息。结果浸润性导管癌及癌旁正常乳腺组织分别获得（1040±15）个蛋白位点和（1003±17）个蛋白位点，差异在2倍以上共有28个蛋白位点，其中22个蛋白点表达上调，6个蛋白点表达下调，有23个蛋白点得到鉴定（蛋白得分大于70分），其中得分最高的为热休克蛋白27、70、90。结论热休克蛋白27、70、90在乳腺浸润性导管癌组织中呈现高表达状态，提示它们与乳腺癌密切相关，有可能成为乳腺浸润性导管癌的联合特异性标记物。
目的：篩選乳腺癌組織與癌徬正常乳腺組織之間的差異蛋白，尋找和鑒定與乳腺癌相關的蛋白質。方法選取新鮮乳腺浸潤性導管癌組織及癌徬正常乳腺組織行雙嚮凝膠電泳，得到的差異蛋白點進行圖像分析，運用質譜分析對所篩選的差異蛋白點做質譜鑒定，根據檢索軟件Mascot計算得到蛋白的分值，輸入NCBI數據庫來穫得蛋白質的信息。結果浸潤性導管癌及癌徬正常乳腺組織分彆穫得（1040±15）箇蛋白位點和（1003±17）箇蛋白位點，差異在2倍以上共有28箇蛋白位點，其中22箇蛋白點錶達上調，6箇蛋白點錶達下調，有23箇蛋白點得到鑒定（蛋白得分大于70分），其中得分最高的為熱休剋蛋白27、70、90。結論熱休剋蛋白27、70、90在乳腺浸潤性導管癌組織中呈現高錶達狀態，提示它們與乳腺癌密切相關，有可能成為乳腺浸潤性導管癌的聯閤特異性標記物。
목적：사선유선암조직여암방정상유선조직지간적차이단백，심조화감정여유선암상관적단백질。방법선취신선유선침윤성도관암조직급암방정상유선조직행쌍향응효전영，득도적차이단백점진행도상분석，운용질보분석대소사선적차이단백점주질보감정，근거검색연건Mascot계산득도단백적분치，수입NCBI수거고래획득단백질적신식。결과침윤성도관암급암방정상유선조직분별획득（1040±15）개단백위점화（1003±17）개단백위점，차이재2배이상공유28개단백위점，기중22개단백점표체상조，6개단백점표체하조，유23개단백점득도감정（단백득분대우70분），기중득분최고적위열휴극단백27、70、90。결론열휴극단백27、70、90재유선침윤성도관암조직중정현고표체상태，제시타문여유선암밀절상관，유가능성위유선침윤성도관암적연합특이성표기물。
ObjectiveTo screen differential proteins between breast cancer tissues and adjacent normal breast tissues, and to search and identify proteins associated with breast cancer.Methods Proteins were extracted from fresh breast invasive ductal carcinoma and adjacent normal breast tissues. The two-dimensional gel electrophoresis was performed then protein spots were obtained and analyzed by image analysis. The differential protein spots were identified using mass spectrometry and the scores of proteins were calculated with retrieval software Mascot then were inputted into NCBI protein database in order to obtain information.Results(1 040±15) protein spots and (1 003±17) protein spots were obtained from invasive ductal carcinoma and adjacent normal breast tissues respectively. There were 28 protein spots with significant differences up to 2 fold between cancer and normal tissues, of which 22 protein spots up-regulated, 6 down-regulated and 23 protein spots identified whose protein scores were more than 70 points. Notably the highest ones were identified as heat shock protein 27, 70, 90.Conclusion Heat shock proteins 27, 70, 90 exhibit strong expression in invasive ductal breast carcinoma, suggesting that they are closely related to breast cancer, and may become a joint tumor-specific marker of breast invasive ductal carcinoma.
