CAJ | 학술논문

目的：研究骨桥蛋白在青年、中年、健康老年人及存在骨质疏松老年人中血清中含量改变，探讨其增龄性变化及在骨质疏松患者中的作用。方法随机单盲法收集太和医院干部病房健康青年、中年、无骨质疏松组老年人及存在骨质疏松老年人血清标本各20份（各组均以骨密度测定结果作为入组标准），收集所有标本电解质（主要为钙、磷值）、骨密度值，并进行Westernblot法检测其中所有标本中骨桥蛋白的表达。结果青中年组电解质中钙、磷值均正常范围，老年组两组中钙、磷值波动较大，有正常范围亦有明显偏低者，与骨密度值无明显相关性。在Westernblot实验中，骨桥蛋白在健康老年组和患病老年组中呈阳性表达，而在青中年组呈阴性，具有明显统计学意义（P＜0．05）；骨桥蛋白在老年组和骨质疏松老年组之间的表达亦存在统计学差异（ P＜0．05）。结论老年组较青中年组中骨桥蛋白量明显增高，提示骨桥蛋白存在增龄性变化。骨桥蛋白含量在骨质疏松老年患者中明显增高，提示骨桥蛋白在人体老化后含量增高，并对骨质代谢造成影响，进而存在某种正向反馈机制，进一步加重骨质疏松症进展。
목적：연구골교단백재청년、중년、건강노년인급존재골질소송노년인중혈청중함량개변，탐토기증령성변화급재골질소송환자중적작용。방법수궤단맹법수집태화의원간부병방건강청년、중년、무골질소송조노년인급존재골질소송노년인혈청표본각20빈（각조균이골밀도측정결과작위입조표준），수집소유표본전해질（주요위개、린치）、골밀도치，병진행Westernblot법검측기중소유표본중골교단백적표체。결과청중년조전해질중개、린치균정상범위，노년조량조중개、린치파동교대，유정상범위역유명현편저자，여골밀도치무명현상관성。재Westernblot실험중，골교단백재건강노년조화환병노년조중정양성표체，이재청중년조정음성，구유명현통계학의의（P＜0．05）；골교단백재노년조화골질소송노년조지간적표체역존재통계학차이（ P＜0．05）。결론노년조교청중년조중골교단백량명현증고，제시골교단백존재증령성변화。골교단백함량재골질소송노년환자중명현증고，제시골교단백재인체노화후함량증고，병대골질대사조성영향，진이존재모충정향반궤궤제，진일보가중골질소송증진전。
Objectives To observe the expression of Osteopontin ( OPN) in plasma of four different groups( youth group,middle aged group,senior aged group without osteoporosis and senior aged group with osteoporosis) .Methods Samples of plasma are all collected from Taihe hospital.20 cases from youth group,20cases from middle aged group 20cases from senior aged group without os-teoporosis and and 20 from senior aged group with osteoporosis.Collect the results of serum electrolytes(mainly calcium and phos-phorus ) and the result of bone mineral density scan.Detected the expression of osteopontin in all samples by Westernblot.Results Calcuim and phosphorus levels are within normal range in youth group and middle aged group.In senior aged group the levels are somewhat below normal but not positively related to the result of bone mineral density scan.Conclusions The expression of OPN stayed the same until humans reach their senior age and it plays an important role in elderly osteoporosis.