CAJ | 학술논문

利用24对 EST-SSR 引物对祁门种群体66个单株和安徽1号进行遗传多样性和亲缘关系分析。结果表明：祁门种群体具有相对较高的遗传多样性，24对引物共检测到等位位点69个，平均2.88个；引物多态性含量 PIC 为0.18~0.85，平均值为0.47；杂合度观测值（Ho）在0.09~0.96之间，平均值为0.41；杂合度期望值（He）在0.23~0.76之间，平均值为0.50；群体内的 Shannon 指数 I 为0.39~1.50，平均0.89。根据相似系数矩阵按 UPGMA 法进行聚类，在相似系数平均值0.50处可将参试的67份材料划分为六大类，其中第Ⅰ类含有44个祁门种群体单株及安徽1号，占总数的67.16%，是祁门种的核心类群。
이용24대 EST-SSR 인물대기문충군체66개단주화안휘1호진행유전다양성화친연관계분석。결과표명：기문충군체구유상대교고적유전다양성，24대인물공검측도등위위점69개，평균2.88개；인물다태성함량 PIC 위0.18~0.85，평균치위0.47；잡합도관측치（Ho）재0.09~0.96지간，평균치위0.41；잡합도기망치（He）재0.23~0.76지간，평균치위0.50；군체내적 Shannon 지수 I 위0.39~1.50，평균0.89。근거상사계수구진안 UPGMA 법진행취류，재상사계수평균치0.50처가장삼시적67빈재료화분위륙대류，기중제Ⅰ류함유44개기문충군체단주급안휘1호，점총수적67.16%，시기문충적핵심류군。
The 24 pairs of EST-SSR primers were used to analyze the genetic polymorphism and genetic relationship of 66 Qimen plants and ‘Anhui 1’. It was showed that the level of genetic diversity within Qimen population was high, with 69 alleles amplified by the 24 EST-SSR primers averaged 2.88. The polymorphism information content (PIC) was ranged from 0.18 to 0.85, with a mean 0.47. The observed heterozygosity (Ho) varied from 0.09 to 0.96, and the expected heterozygosity (He) varied from 0.23 to 0.76, with average of 0.41 and 0.50, respectively. The Shannon index of the Qimen plant population ranged from 0.39 to 1.50, with an average of 0.89. The 66 Qimen plant individuals and cultivar ‘Anhui 1’ were classified into 6 groups based on the UPGMA method with the similarity coefficient at 0.50, and the group I contained 44 Qimen individuals and ‘Anhui 1’, accounted for the 67.16% of the total, which is considered to be core group of the Qimen population.