中国全科医学
中國全科醫學
중국전과의학
CHINESE GENERAL PRACTICE
2015年
20期
2475-2478
,共4页
袁静泊%黄先玫%郑绪阳%韩勇%李小莉
袁靜泊%黃先玫%鄭緒暘%韓勇%李小莉
원정박%황선매%정서양%한용%리소리
产前营养生理学现象%肥胖症%模型,动物
產前營養生理學現象%肥胖癥%模型,動物
산전영양생이학현상%비반증%모형,동물
Prenatal nutritional physiological phenomena%Obesity%Models,animal
目的：建立一种能够研究营养性肥胖妊娠妇女对子代肥胖影响的孕鼠肥胖模型。方法选取SPF级SD成年大鼠，将成功受孕孕鼠40只采用随机数字表法分为对照组（妊娠期间喂养基础饲料），全孕期高能饮食组（妊娠期间喂养高能模型饲料），孕早期高能饮食组（妊娠后前10 d喂养高能模型饲料，之后喂养基础饲料）和高能饮食组（妊娠开始喂养基础饲料，后10 d喂养高能模型饲料），每组各10只。在子代鼠出生后第4、9周，停止喂食12 h后，称取子代鼠体质量，量取体长，计算Lee's指数；在子代鼠股动脉采集0.5 ml血液标本，采用放射免疫分析法检测血糖、血脂、血胰岛素等指标，并计算胰岛素敏感指数（ ISI）；取子代鼠肾周和睾周脂肪组织，计算脂体比。采用不同的标准判断各组致肥率：（1）以超过对照组体质量20%为标准进行判断；（2）以超过对照组脂肪垫重量2倍标准差为标准进行判断；（3）以超过对照组Lee's指数2倍标准差为标准进行判断。结果出生后第9周，4组体质量、体脂肪、脂体比和Lee's指数比较，差异均有统计学意义（ P<0.05）；其中全孕期高能饮食组和孕早期高能饮食组体质量、体脂肪、脂体比和Lee's指数均高于对照组（P<0.05）。以标准（1）进行判断，孕早期高能饮食组在子代鼠出生后第4周和第9周致肥率均为60%；全孕期高能饮食组在子代鼠出生后第4周和第9周致肥率均为70%；以标准（2）进行判断，孕早期高能饮食组在子代鼠出生后第4周和第9周致肥率均为70%；全孕期高能饮食组在子代鼠出生后第4周和第9周致肥率均为80%；以标准（3）进行判断，孕早期高能饮食组在子代鼠出生后第4周和第9周致肥率均为80%；全孕期高能饮食组在子代鼠出生后第4周和第9周致肥率均为90%。出生后第4周，各组血糖、血脂比较，差异无统计学意义（P>0.05）；各组血胰岛素、ISI比较，差异均有统计学意义（P<0.05）；其中全孕期高能饮食组和孕早期高能饮食组血胰岛素和ISI与对照组比较，差异均有统计学意义（ P<0.05）。出生后第9周，各组血胰岛素、ISI和血脂比较，差异均有统计学意义（ P<0.05），其中全孕期高能饮食组和孕早期高能饮食组血胰岛素、ISI和血脂与对照组比较，差异均有统计学意义（ P<0.05）。出生后第9周，各组肝脏、脂肪组织重量比较，差异均有统计学意义（P<0.05）；其中全孕期高能饮食组和孕早期高能饮食组肝脏和脂肪组织重量较对照组增加（P<0.05）。结论通过调整孕鼠饮食数量及时间，出生后第4周可诱导建立营养性肥胖孕鼠对子代鼠肥胖影响模型，出生后第9周可建立营养性孕鼠对子代鼠肥胖和代谢综合征影响模型。
目的：建立一種能夠研究營養性肥胖妊娠婦女對子代肥胖影響的孕鼠肥胖模型。方法選取SPF級SD成年大鼠，將成功受孕孕鼠40隻採用隨機數字錶法分為對照組（妊娠期間餵養基礎飼料），全孕期高能飲食組（妊娠期間餵養高能模型飼料），孕早期高能飲食組（妊娠後前10 d餵養高能模型飼料，之後餵養基礎飼料）和高能飲食組（妊娠開始餵養基礎飼料，後10 d餵養高能模型飼料），每組各10隻。在子代鼠齣生後第4、9週，停止餵食12 h後，稱取子代鼠體質量，量取體長，計算Lee's指數；在子代鼠股動脈採集0.5 ml血液標本，採用放射免疫分析法檢測血糖、血脂、血胰島素等指標，併計算胰島素敏感指數（ ISI）；取子代鼠腎週和睪週脂肪組織，計算脂體比。採用不同的標準判斷各組緻肥率：（1）以超過對照組體質量20%為標準進行判斷；（2）以超過對照組脂肪墊重量2倍標準差為標準進行判斷；（3）以超過對照組Lee's指數2倍標準差為標準進行判斷。結果齣生後第9週，4組體質量、體脂肪、脂體比和Lee's指數比較，差異均有統計學意義（ P<0.05）；其中全孕期高能飲食組和孕早期高能飲食組體質量、體脂肪、脂體比和Lee's指數均高于對照組（P<0.05）。以標準（1）進行判斷，孕早期高能飲食組在子代鼠齣生後第4週和第9週緻肥率均為60%；全孕期高能飲食組在子代鼠齣生後第4週和第9週緻肥率均為70%；以標準（2）進行判斷，孕早期高能飲食組在子代鼠齣生後第4週和第9週緻肥率均為70%；全孕期高能飲食組在子代鼠齣生後第4週和第9週緻肥率均為80%；以標準（3）進行判斷，孕早期高能飲食組在子代鼠齣生後第4週和第9週緻肥率均為80%；全孕期高能飲食組在子代鼠齣生後第4週和第9週緻肥率均為90%。齣生後第4週，各組血糖、血脂比較，差異無統計學意義（P>0.05）；各組血胰島素、ISI比較，差異均有統計學意義（P<0.05）；其中全孕期高能飲食組和孕早期高能飲食組血胰島素和ISI與對照組比較，差異均有統計學意義（ P<0.05）。齣生後第9週，各組血胰島素、ISI和血脂比較，差異均有統計學意義（ P<0.05），其中全孕期高能飲食組和孕早期高能飲食組血胰島素、ISI和血脂與對照組比較，差異均有統計學意義（ P<0.05）。齣生後第9週，各組肝髒、脂肪組織重量比較，差異均有統計學意義（P<0.05）；其中全孕期高能飲食組和孕早期高能飲食組肝髒和脂肪組織重量較對照組增加（P<0.05）。結論通過調整孕鼠飲食數量及時間，齣生後第4週可誘導建立營養性肥胖孕鼠對子代鼠肥胖影響模型，齣生後第9週可建立營養性孕鼠對子代鼠肥胖和代謝綜閤徵影響模型。
목적：건립일충능구연구영양성비반임신부녀대자대비반영향적잉서비반모형。방법선취SPF급SD성년대서，장성공수잉잉서40지채용수궤수자표법분위대조조（임신기간위양기출사료），전잉기고능음식조（임신기간위양고능모형사료），잉조기고능음식조（임신후전10 d위양고능모형사료，지후위양기출사료）화고능음식조（임신개시위양기출사료，후10 d위양고능모형사료），매조각10지。재자대서출생후제4、9주，정지위식12 h후，칭취자대서체질량，량취체장，계산Lee's지수；재자대서고동맥채집0.5 ml혈액표본，채용방사면역분석법검측혈당、혈지、혈이도소등지표，병계산이도소민감지수（ ISI）；취자대서신주화고주지방조직，계산지체비。채용불동적표준판단각조치비솔：（1）이초과대조조체질량20%위표준진행판단；（2）이초과대조조지방점중량2배표준차위표준진행판단；（3）이초과대조조Lee's지수2배표준차위표준진행판단。결과출생후제9주，4조체질량、체지방、지체비화Lee's지수비교，차이균유통계학의의（ P<0.05）；기중전잉기고능음식조화잉조기고능음식조체질량、체지방、지체비화Lee's지수균고우대조조（P<0.05）。이표준（1）진행판단，잉조기고능음식조재자대서출생후제4주화제9주치비솔균위60%；전잉기고능음식조재자대서출생후제4주화제9주치비솔균위70%；이표준（2）진행판단，잉조기고능음식조재자대서출생후제4주화제9주치비솔균위70%；전잉기고능음식조재자대서출생후제4주화제9주치비솔균위80%；이표준（3）진행판단，잉조기고능음식조재자대서출생후제4주화제9주치비솔균위80%；전잉기고능음식조재자대서출생후제4주화제9주치비솔균위90%。출생후제4주，각조혈당、혈지비교，차이무통계학의의（P>0.05）；각조혈이도소、ISI비교，차이균유통계학의의（P<0.05）；기중전잉기고능음식조화잉조기고능음식조혈이도소화ISI여대조조비교，차이균유통계학의의（ P<0.05）。출생후제9주，각조혈이도소、ISI화혈지비교，차이균유통계학의의（ P<0.05），기중전잉기고능음식조화잉조기고능음식조혈이도소、ISI화혈지여대조조비교，차이균유통계학의의（ P<0.05）。출생후제9주，각조간장、지방조직중량비교，차이균유통계학의의（P<0.05）；기중전잉기고능음식조화잉조기고능음식조간장화지방조직중량교대조조증가（P<0.05）。결론통과조정잉서음식수량급시간，출생후제4주가유도건립영양성비반잉서대자대서비반영향모형，출생후제9주가건립영양성잉서대자대서비반화대사종합정영향모형。
Objective To establish a model of pregnant rats obesity which can be used to study the influence of pregnant women obesity over offspring obesity. Methods 40 adult SD rats of SPF level being pregnant successfully were, according to random number table,divided into control group( being fed with basic diet),high-energy diet group during whole pregnancy(being fed with high energy model diet),high-energy diet group during early pregnancy(being fed with high-energy diet 10 d after pregnancy and afterwards basic diet) and high-energy diet group in late pregnancy( being fed with basic diet and 10 d before delivery high-energy diet). Every group had 10 rats. The body weight,body length and Lee's index of the offspring rats were measured 12 h after stopping feeding when the offspring rats age were 4 and 9 weeks;0. 5 ml blood sample were collected from the femoral artery to measure blood glucose, blood lipids, insulin and other related indicators by radioimmunoassay method and calculate insulin sensitivity index ( ISI );fat tissue around kidney and testicle was used to calculate the rate of fat mass. Different standards were used the judge the rate of causing fat:(1) the body mass in each group was 20% higher than that in the control group;(2) the standard difference of the fat pad in each group and that in the control group was over 2;(3) the standard difference of Lee's index between them was over 2. Results At postnatal 9 weeks,there was statistically significant difference in body weight,body fat,Lee's index(P<0. 05),which were all higher in high-energy diet group during whole pregnancy than those in high -energy diet group during early pregnancy ( P <0. 05 ). The rates of causing fat at postnatal 4 weeks and 9 weeks were all 60% in high energy diet group during early pregnancy and 70% in high energy diet group during the whole pregnancy according to the first standard;which were 70% and 80% respectively according to the second standard and 80% and 90% respectively according to the third standard. At postnatal 4 weeks,the level of blood glucose and lipids showed no significant differences between the groups(P>0. 05),however the level of insulin secretion and insulin sensitivity index between the groups was found significant difference(P<0. 05)and that difference between high energy diet group during the whole pregnancy and high energy diet group during the early pregnancy was also significant(P<0. 05). At postnatal 9 weeks,the level of lipids,ISI and insulin secretion showed no significant differences between the groups(P<0. 05) and those differences were also significant between high energy diet group during the whole pregnancy and high energy diet group during the early pregnancy(P<0. 05). At postnatal 9 weeks the weights of liver and fat tissue in each group were statistically significant(P<0. 05),and the weights of liver and fat tissue in high energy diet groups during the whole pregnancy and during the early pregnancy were heavier than those in the control group ( P<0. 05 ). Conclusion By adjusting the number and the time of pregnancy rats, the obesity model of pregnancy nutrition - induced offspring obesity can be induced to establish at postnatal 4 weeks,and the obesity and metabolic syndrome rat model can be established at postnatal 9 weeks.
