CAJ | 학술논문

目的：探讨CD36基因rs17154181、rs1761667位点多态性与2型糖尿病患者发生大血管病变的关系。方法选取2012年12月—2014年6月于广西医科大学第一附属医院内分泌代谢科住院治疗的2型糖尿病患者112例为病例组，并选取同期于本院体检健康者65例为对照组。记录两组一般资料，并检测相关生化指标。提取外周血DNA，CD36基因rs17154181和rs1761667经PCR扩增后分析基因型。结果两组CD36基因rs17154181、rs1761667位点基因型和等位基因频率比较，差异无统计学意义（ P>0.05）。病例组不同程度糖尿病大血管病变患者年龄、总胆固醇（TC）、三酰甘油（TG）水平比较，差异有统计学意义（P<0.05）；其中，临床动脉粥样硬化和亚临床动脉粥样硬化患者年龄大于无大血管病变患者，TC水平低于无大血管病变患者；亚临床动脉粥样硬化患者TG水平低于无大血管病变患者（P<0.05）。病例组不同程度糖尿病大血管病变患者CD36基因rs17154181、rs1761667位点基因型及等位基因频率比较，差异均无统计学意义（ P>0.05）。病例组rs17154181位点不同基因型患者收缩压比较，差异有统计学意义（P<0.05），其中，基因型AG患者收缩压高于基因型AA患者（P<0.05）。多因素Logistic回归分析显示，年龄、合并高血压、低密度脂蛋白胆固醇（ LDL-C）进入回归方程，是2型糖尿病患者发生糖尿病大血管病变的影响因素（P<0.05），而 rs17154181、rs1761667位点基因型未进入回归方程（ P >0.05）。结论本研究未发现 CD36基因rs17154181、rs1761667位点多态性与广西地区2型糖尿病患者发生大血管病变有关，其多态性可能不是CD36与糖脂代谢有关的功能性多态位点。
목적：탐토CD36기인rs17154181、rs1761667위점다태성여2형당뇨병환자발생대혈관병변적관계。방법선취2012년12월—2014년6월우엄서의과대학제일부속의원내분비대사과주원치료적2형당뇨병환자112례위병례조，병선취동기우본원체검건강자65례위대조조。기록량조일반자료，병검측상관생화지표。제취외주혈DNA，CD36기인rs17154181화rs1761667경PCR확증후분석기인형。결과량조CD36기인rs17154181、rs1761667위점기인형화등위기인빈솔비교，차이무통계학의의（ P>0.05）。병례조불동정도당뇨병대혈관병변환자년령、총담고순（TC）、삼선감유（TG）수평비교，차이유통계학의의（P<0.05）；기중，림상동맥죽양경화화아림상동맥죽양경화환자년령대우무대혈관병변환자，TC수평저우무대혈관병변환자；아림상동맥죽양경화환자TG수평저우무대혈관병변환자（P<0.05）。병례조불동정도당뇨병대혈관병변환자CD36기인rs17154181、rs1761667위점기인형급등위기인빈솔비교，차이균무통계학의의（ P>0.05）。병례조rs17154181위점불동기인형환자수축압비교，차이유통계학의의（P<0.05），기중，기인형AG환자수축압고우기인형AA환자（P<0.05）。다인소Logistic회귀분석현시，년령、합병고혈압、저밀도지단백담고순（ LDL-C）진입회귀방정，시2형당뇨병환자발생당뇨병대혈관병변적영향인소（P<0.05），이 rs17154181、rs1761667위점기인형미진입회귀방정（ P >0.05）。결론본연구미발현 CD36기인rs17154181、rs1761667위점다태성여엄서지구2형당뇨병환자발생대혈관병변유관，기다태성가능불시CD36여당지대사유관적공능성다태위점。
Objective To investigate the relationship between the polymorphism of rs17154181 and rs1761667 sites of CD36 gene and macroangiopathy in patients with type 2 diabetes mellitus in Guangxi Province. Methods We enrolled 112 patients with type 2 diabetes mellitus who received hospitalized treatment in the Department of Endocrinology and Metabolism of the First Affiliated Hospital of Guangxi Medical University from December 2012 to June 2014 as the case group. Another 65 healthy people who underwent physical examination in the same period were enrolled as control group. General data were recorded,and relevant biochemical indicators were tested. DNA in peripheral blood was extracted , and through PCR amplification of rs17154181 and rs1761667 sites of CD36 gene,genotypes were determined. Results The two groups were not significantly different(P>0. 05)in the distribution of genotypes and allele frequency at rs17154181 and rs1761667 sites of CD36 gene. In the case group,the diabetic patients with different levels of macroangiopathy were significantly different( P<0. 05)in age,TC and TG;patients with clinical arteriosclerosis and subclinical arteriosclerosis were higher(P<0. 05)in age and were lower ( P<0. 05 )in TC level than the diabetic patients without macroangiopathy;diabetic patients with subclinical arteriosclerosis were lower(P<0. 05)than the diabetic patients without macroangiopathy in TG level. In the case group,the diabetic patients with different levels of macroangiopathy were not significantly different ( P>0. 05 )in the rs 17154181 and rs 1761667 genotypes of CD36 genes and allele frequency. In the case group,patients with different genotypes of rs17154181 site were significantly different(P<0. 05)in systolic pressure;patients with AG genotype were higher(P<0. 05)than patients with AA genotype in systolic pressure. The multivariate logistic regression analysis showed that age,complicated hypertension and LDL-C entered the regression equation,which indicated that these factors were the influencing factors for macroangiopathy in patients with type 2 diabetes mellitus. The genotypes at rs17154181 and rs1761667 sites didn't enter the regression equation (P>0. 05). Conclusion No correlation was found between the polymorphisms of rs17154181 and rs1761667 sites of CD36 gene and macroangiopathy in patients with type 2 diabetes mellitus. The two sites may be not the CD36 functional polymorphism sites relevant with glucolipid metabolism.