河南科学
河南科學
하남과학
HENAN SCIENCE
2015年
8期
1329-1332
,共4页
杨攀%王升%郭风民%李永华
楊攀%王升%郭風民%李永華
양반%왕승%곽풍민%리영화
月季%蔷薇%花粉生活力%沙尔达考夫染色%悬滴液发芽法
月季%薔薇%花粉生活力%沙爾達攷伕染色%懸滴液髮芽法
월계%장미%화분생활력%사이체고부염색%현적액발아법
Rosa Chinensis%Rosa multiflora%pollen viability%staining%germination
以30个月季品种及蔷薇属植物为试验材料,采用沙尔达考夫染色法、悬滴液发芽法测定其花粉生活力,并根据花粉萌发率对品种进行分级.结果表明:1)悬滴液发芽法更适合花粉生活力的测定,10%蔗糖浓度下适宜月季品种及蔷薇属植物的花粉萌发;2)30个月季品种和蔷薇中,花粉萌发率较高的是‘十姊妹’‘、橘红火焰’‘、小果蔷薇’和‘粉荷’,分别为84.20%、71.60%、59.20%和54.00%,花粉生活力随着时间的延长而降低;3)以花粉萌发率为判断标准,月季品种及蔷薇分为三个等级,一级品种:‘十姊妹’、‘橘红火焰’,萌发率高于50%;二级品种:‘粉和平’、‘摩尼卡’,萌发率在10%~50%;三级品种:‘光谱’、‘金阵雨’,萌发率低于10%.
以30箇月季品種及薔薇屬植物為試驗材料,採用沙爾達攷伕染色法、懸滴液髮芽法測定其花粉生活力,併根據花粉萌髮率對品種進行分級.結果錶明:1)懸滴液髮芽法更適閤花粉生活力的測定,10%蔗糖濃度下適宜月季品種及薔薇屬植物的花粉萌髮;2)30箇月季品種和薔薇中,花粉萌髮率較高的是‘十姊妹’‘、橘紅火燄’‘、小果薔薇’和‘粉荷’,分彆為84.20%、71.60%、59.20%和54.00%,花粉生活力隨著時間的延長而降低;3)以花粉萌髮率為判斷標準,月季品種及薔薇分為三箇等級,一級品種:‘十姊妹’、‘橘紅火燄’,萌髮率高于50%;二級品種:‘粉和平’、‘摩尼卡’,萌髮率在10%~50%;三級品種:‘光譜’、‘金陣雨’,萌髮率低于10%.
이30개월계품충급장미속식물위시험재료,채용사이체고부염색법、현적액발아법측정기화분생활력,병근거화분맹발솔대품충진행분급.결과표명:1)현적액발아법경괄합화분생활력적측정,10%자당농도하괄의월계품충급장미속식물적화분맹발;2)30개월계품충화장미중,화분맹발솔교고적시‘십자매’‘、귤홍화염’‘、소과장미’화‘분하’,분별위84.20%、71.60%、59.20%화54.00%,화분생활력수착시간적연장이강저;3)이화분맹발솔위판단표준,월계품충급장미분위삼개등급,일급품충:‘십자매’、‘귤홍화염’,맹발솔고우50%;이급품충:‘분화평’、‘마니잡’,맹발솔재10%~50%;삼급품충:‘광보’、‘금진우’,맹발솔저우10%.
With a total of 30 Rosa chinensis varieties and Rosa multiflora as test materials,the pollen viability was measured by staining and germination,and the varieties was graded according to the pollen germination. The result showed as follows. The germination was suitable for detecting pollen viability,the measurement with 10%sucrose concentration was suitable for the Rosa chinensis and Rosa multiflora varieties pollen germination.‘Rosa multiflora Thunb.var.platyphylla Thory’‘Darthuizer Orang Fire’‘Rosa cymos’and‘Fen He’had the higher pollen germination rate than other varieties,and the rates respectively were 84.20%,71.60%,59.20%and 54.00%,the pollen viability gradually reduced as the extension of storage time. With the pollen germination as the judgment ,Rosa chinensis and Rosa multiflora varieties were classified three grades:the pollen germination of the first grade was more than 50%,‘Rosa multiflora Thunb.var.platyphylla Thory’‘Darthuizer Orang Fire’;the second grade was between 10% and 50%‘,Pink Peace‘’Monica’;the third grade was less than 10%‘,Spectra‘’ Golden Showers’.