CAJ | 학술논문

目的：如何获得高质量、数量充足的小鼠胰岛细胞。方法通过比较当前研究中使用的大多数消化酶来分离小鼠胰岛，证实相继使用XI型胶原酶和Dispase比单独使用前者更为有效。结果可获得更多的胰岛细胞（增加至40％）、更短的消化时间（下降25％），并且使用较少的胶原酶（减少40％）。结论这一新改良的方案在小鼠胰岛分离的质量、数量方面都有明显改善。
목적：여하획득고질량、수량충족적소서이도세포。방법통과비교당전연구중사용적대다수소화매래분리소서이도，증실상계사용XI형효원매화Dispase비단독사용전자경위유효。결과가획득경다적이도세포（증가지40％）、경단적소화시간（하강25％），병차사용교소적효원매（감소40％）。결론저일신개량적방안재소서이도분리적질량、수량방면도유명현개선。
Objective To adequate amount of islets with good quality were necessary for a reliable investigation . Mouse pancreatic islets were widely used in diabetes‐related studies .Method The enzymes that were used for diges‐ting redundant tissue around islets played an essential part in the process of the isolation .Through a comparative study with most currently used digestive enzymes ,the sequential application of type XI collagenase and dispase ,in‐stead of using the former alone ,was verified as the most effective scheme for mouse islet isolation .Result High islet yield(40% increase) ,short digestive time(25% decrease)and less collagenase(40% decrease)were achieved compa‐ring with those digested by collagenase alone .Conclusion This newly modified protocol demonstrated a significant im‐provement for mouse islet isolation in terms of quantity and quality .