CAJ | 학술논문

目的：研究CAMKK2在前列腺癌及癌旁组织中的表达，分析其表达与临床病理数据的关系。方法通过实时荧光定量PCR技术检测CAMKK2的mRNA水平在前列腺癌及癌旁组织中的表达情况，通过免疫印迹及免疫组化技术检测CAMKK2的蛋白水平在两种组织中的表达，分析CAMKK2的表达水平与前列腺癌相关临床病理特征及预后的关系。结果前列腺癌组织中的CAMKK2在mRNA及蛋白水平上均较癌旁组织中上调表达， CAMKK2的表达与血清PSA水平、是否转移及是否生化复发有关，CAMKK2的表达高低与无生化复发生存率相关，CAM KK2是生化复发的独立危险预测因素。结论 CAM KK2在前列腺癌组织中的表达增加，可能提示患者的预后情况，并可作为前列腺癌新的生物标记物。
목적：연구CAMKK2재전렬선암급암방조직중적표체，분석기표체여림상병리수거적관계。방법통과실시형광정량PCR기술검측CAMKK2적mRNA수평재전렬선암급암방조직중적표체정황，통과면역인적급면역조화기술검측CAMKK2적단백수평재량충조직중적표체，분석CAMKK2적표체수평여전렬선암상관림상병리특정급예후적관계。결과전렬선암조직중적CAMKK2재mRNA급단백수평상균교암방조직중상조표체， CAMKK2적표체여혈청PSA수평、시부전이급시부생화복발유관，CAMKK2적표체고저여무생화복발생존솔상관，CAM KK2시생화복발적독립위험예측인소。결론 CAM KK2재전렬선암조직중적표체증가，가능제시환자적예후정황，병가작위전렬선암신적생물표기물。
Objective To investigate the expression and significances of CAMKK2 in prostate cancer and adjacent non‐cancerous tissues ,and analyze the associations of the expression of CAMKK2 and the clinical pathological fea‐tures .Method Q‐PCR was used to detect the CAMKK2 mRNA level in prostate cancer and adjacent non‐cancerous tissues ,western blot(WB)and immunohistochemistry(IHC)were used to detect the CAMKK2 protein level ,the asso‐ciations of CAMKK2 expression with prostate cancer clinical pathological features and prognosis were analyzed .Re‐sult The mRNA and protein level of CAMKK2 were upregulated in prostate cancer tissues than in adjacent non‐can‐cerous tissues ,the expression of CAMKK2 had relation with serum PSA level ,metastasis and PSA failure ,the ex‐pression of CAMKK2 had relation with biochemical non‐recurrence survival ,and it might be an independent predictor of biochemical recurrence in prostate cancer .Conclusion The upregulation of CAMKK2 in prostate cancer may predict the prognosis of prostate cancer patient ,and it may be a new biomarker of prostate cancer .