CAJ | 학술논문

以12份向日葵杂交种和18份向日葵亲本为材料，利用 SSR 分子标记，从502对 SSR 引物中筛选了34对核心引物。34对引物在30份研究材料中共检测到81个多态性片段，平均每对引物的多态性片段为2.38个。基于34个 SSR 标记的扩增结果，利用 NTsys 2.10e 软件计算遗传相似系数，并建立 UPGMA 聚类图，结果表明：30份材料之间的遗传相似系数变化范围为0.4320～0.9012，在遗传相似系数0.62处可以将30份材料分为两大类，一定程度上反映了材料间的亲缘关系。同时 SSR 标记指纹图谱的建立为向日葵品种纯度和真伪鉴定提供了科学数据。
이12빈향일규잡교충화18빈향일규친본위재료，이용 SSR 분자표기，종502대 SSR 인물중사선료34대핵심인물。34대인물재30빈연구재료중공검측도81개다태성편단，평균매대인물적다태성편단위2.38개。기우34개 SSR 표기적확증결과，이용 NTsys 2.10e 연건계산유전상사계수，병건립 UPGMA 취류도，결과표명：30빈재료지간적유전상사계수변화범위위0.4320～0.9012，재유전상사계수0.62처가이장30빈재료분위량대류，일정정도상반영료재료간적친연관계。동시 SSR 표기지문도보적건립위향일규품충순도화진위감정제공료과학수거。
34 core SSR primers were selected from 502 SSR primers on 12 sunflower hybrids and 18 parental lines used SSR. 81 polymorphic DNA bands were detected by using 34 core SSR primers with averaged 2.38 bands per primer. Ge-netic similarity coefficient was computed using the software NTsys 2.10e, and a dendrogram of genetic relationship was con-structed using UPGMA method. The results indicated that the genetic similarity ranged from 0.432 0~0.901 2 among 30 cultivars. Two cluster groups were classified with the similarity coefficient of 0.62, revealed the genetic relatives among culti-vars. The sunflower SSR construction of DNA fingerprinting provided scientific data for identification as well as determination of purity of the sunflower cultivars.