CAJ | 학술논문

目的：探讨脑缺血再灌注大鼠不同时间碱性成纤维细胞生长因子(bFGF)和血小板源性生长因子-B(PDGF-B)的表达水平及其与血管形成的关系。方法采用线栓法制备大鼠大脑中动脉局灶性脑缺血再灌注(MCAO/R)模型,分为假手术组和 MCAO/R 组,MCAO/R 组缺血2 h后根据再灌注时间窗的不同分为0、6、24 h、3、7、14、21 d共7组,应用 HE 染色观察病理变化并测定脑梗死体积,用免疫组化法检测 CD34蛋白的表达水平并计数微血管密度(MVD),用免疫组化法检测 bFGF 和 PDGF-B 蛋白的表达水平。结果bFGF蛋白表达水平在 MCAO/R 6 h后即开始升高,3 d到达高峰,7 d开始降低。PDGF-B 蛋白表达水平在 MCAO/R 24 h后明显升高,3 d到达高峰,7 d有所下降,持续到14 d左右。MVD 表达在 MCAO/R 3 d开始升高,7 d到达高峰。bFGF 和 PDGF-B 蛋白表达水平与 MVD 变化呈正相关(P <0.01)。结论局灶性脑缺血再灌注损伤可诱导 bFGF、PDGF-B 和新生血管表达增加,激活内源性脑保护机制。
목적：탐토뇌결혈재관주대서불동시간감성성섬유세포생장인자(bFGF)화혈소판원성생장인자-B(PDGF-B)적표체수평급기여혈관형성적관계。방법채용선전법제비대서대뇌중동맥국조성뇌결혈재관주(MCAO/R)모형,분위가수술조화 MCAO/R 조,MCAO/R 조결혈2 h후근거재관주시간창적불동분위0、6、24 h、3、7、14、21 d공7조,응용 HE 염색관찰병리변화병측정뇌경사체적,용면역조화법검측 CD34단백적표체수평병계수미혈관밀도(MVD),용면역조화법검측 bFGF 화 PDGF-B 단백적표체수평。결과bFGF단백표체수평재 MCAO/R 6 h후즉개시승고,3 d도체고봉,7 d개시강저。PDGF-B 단백표체수평재 MCAO/R 24 h후명현승고,3 d도체고봉,7 d유소하강,지속도14 d좌우。MVD 표체재 MCAO/R 3 d개시승고,7 d도체고봉。bFGF 화 PDGF-B 단백표체수평여 MVD 변화정정상관(P <0.01)。결론국조성뇌결혈재관주손상가유도 bFGF、PDGF-B 화신생혈관표체증가,격활내원성뇌보호궤제。
Objective To investigate the effects of angiogenesis and the expression changes of basic fi-broblast growth factor (bFGF)and platelet-derived growth factor B chain(PDGF-B )in rats following focal cerebral ischemia/reperfusion.Methods A model of the middle cerebral artery occlusion 2h / reperfusion (MCAO/R)in rats was performed with the intraluminal filament occlusion.Rats were divided into sham oper-ation group and MCAO/R group.Two hours after ischemia the MCAO/R group was divided into 7 subgroups according to reperfusion time difference,including 0,6,24 h,3,7,14,21 d.Brain sections were stained with hematoxylin and eosin (HE)for surveying the volume of infraction.The expression of microvascular den-sity(MVD)was determined by immunohistochemical staining.The expression of bFGF and PDGF-B protein were determined by mmunohistochemical staining.Results The expressions of bFGF began to increase at 6 h after ischemia/reperfusion.It reached climax at 3 d and reduced after 7 d.The expressions of PDGF-B were significantly increased at 24 h after ischemia/reperfusion,with the first peak at 3 d and the second peak was at 14 d.The expressions of MVD began to increase at 3 d after ischemia/reperfusion and continue to 14 d.There was a significant positive correlation between expression of bFGF,PDGF-B and change of MVD(P <0.05 ). Conclusions Ischemia and reperfusion injury can induce expression of bFGF,PDGF-B and angiogenesis, which might play an important role in the internal protection of neurons.