CAJ | 학술논문

DREB 类转录因子在作物抵御逆境胁迫上起重要作用，利用该类基因改良作物抗逆性具有重要意义。研究利用同源克隆方法在6份向日葵资源中均分离到1个 DREB 类转录因子基因。该基因序列全长945 bp，推测编码蛋白含314个氨基酸，与 NCBI 中该基因序列同源性均达到99%以上，不同材料之间该基因序列存在单碱基突变，突变位置不一致，且未发现与耐旱性有明显相关性。表达分析显示，6份向日葵材料根、茎和叶中的 DREB 基因均受干旱、盐害和低温的诱导，作出表达量上调的应答，胁迫超过一定时间，表达量出现下降的趋势。所有材料叶中 DREB 基因的上调幅度明显高于根和茎中，低温胁迫后的表达上调量要明显低于干旱和盐害胁迫下的表达上调量。各材料根、茎、叶中 DREB 的表达量与耐旱性未发现有明显相关性。
DREB 류전록인자재작물저어역경협박상기중요작용，이용해류기인개량작물항역성구유중요의의。연구이용동원극륭방법재6빈향일규자원중균분리도1개 DREB 류전록인자기인。해기인서렬전장945 bp，추측편마단백함314개안기산，여 NCBI 중해기인서렬동원성균체도99%이상，불동재료지간해기인서렬존재단감기돌변，돌변위치불일치，차미발현여내한성유명현상관성。표체분석현시，6빈향일규재료근、경화협중적 DREB 기인균수간한、염해화저온적유도，작출표체량상조적응답，협박초과일정시간，표체량출현하강적추세。소유재료협중 DREB 기인적상조폭도명현고우근화경중，저온협박후적표체상조량요명현저우간한화염해협박하적표체상조량。각재료근、경、협중 DREB 적표체량여내한성미발현유명현상관성。
DREB transcription factors play an important role in the plant stress tolerance, it has important significance to gain stress-tolerant crops by transgenic technologies. In the study, a DREB-like gene was cloned from 6 sunflower germplasms. The DREB cDNA was 945 bp in length, and encoded protein of 314 amino acids which shared 99% similarity with DREB registered in NCBI. There were 5 single base mutations in 4 germplams, in which only one make amino acid mutation. No significant correlation of SNP with drought tolerance was found in this study. Expression analysis showed that DREB genes in leaves/stems and root in all six germplasms made a response by an increased expression up-regulation due to induction of drought, salinity and low temperature stress and the expression decreased when the stress exceeded a certain time. The up regulation of DREB gene in the leaves of all materials was significantly higher than those of the roots and stems, and the up-regulated expression after chilling stress was significantly lower than those of the drought and salt stress. The expres-sion of DREB in root, stem and leaf of each material was not significantly correlated with the tolerance to drought.