CAJ | 학술논문

目的探讨microRNA-34c-5p（miR-34c）在喉癌前病变组织中差异表达情况，并进行临床病理相关性分析。方法按照2005年世界卫生组织（WHO）病理诊断标准，94例喉癌前病变分为轻度异型增生、中度异型增生、重度异型增生及原位癌；实时定量聚合酶链式反应（quantitative real-time polymerase chain reaction，qRT-PCR）方法检测94例喉癌前病变组织中miR-34c在各组间的差异表达情况，并结合临床资料及随访结果分析miR-34c与喉癌前病变病例临床因素的相关性。结果94例喉癌前病变病例年龄37~86岁，平均58岁，男性85例（90.4%），女性9例（9.6%）。轻度异型增生组20例（21.3%），中度异型增生组23例（24.5%），重度异型增生组26例（27.7%），原位癌组25例（26.6%）。随喉癌前病变级别增高，miR-34c在喉癌前病变组织中呈上调表达趋势，总体组间存在统计学差异（F=10.182，P=0.00），而在浸润性鳞状细胞癌组下降，甚至明显低于正常鳞状上皮组织的表达均值。临床病理分析显示饮酒病例中表达明显高于非饮酒病例，且miR-34c与喉癌前病变患者饮酒因素存在明显线性依存关系（r =0.31，P=0.03）。结论MiR-34c在喉癌前病变组织中的上调表达趋势，可能为辅助喉癌前病变病理分级和早期喉癌诊断提供一定的分子病理参考价值，miR-34c的上调表达与喉癌前病变饮酒因素呈线性相关，对于指导喉癌前病变病例的临床管理可能具有重要临床意义。
목적탐토microRNA-34c-5p（miR-34c）재후암전병변조직중차이표체정황，병진행림상병리상관성분석。방법안조2005년세계위생조직（WHO）병리진단표준，94례후암전병변분위경도이형증생、중도이형증생、중도이형증생급원위암；실시정량취합매련식반응（quantitative real-time polymerase chain reaction，qRT-PCR）방법검측94례후암전병변조직중miR-34c재각조간적차이표체정황，병결합림상자료급수방결과분석miR-34c여후암전병변병례림상인소적상관성。결과94례후암전병변병례년령37~86세，평균58세，남성85례（90.4%），녀성9례（9.6%）。경도이형증생조20례（21.3%），중도이형증생조23례（24.5%），중도이형증생조26례（27.7%），원위암조25례（26.6%）。수후암전병변급별증고，miR-34c재후암전병변조직중정상조표체추세，총체조간존재통계학차이（F=10.182，P=0.00），이재침윤성린상세포암조하강，심지명현저우정상린상상피조직적표체균치。림상병리분석현시음주병례중표체명현고우비음주병례，차miR-34c여후암전병변환자음주인소존재명현선성의존관계（r =0.31，P=0.03）。결론MiR-34c재후암전병변조직중적상조표체추세，가능위보조후암전병변병리분급화조기후암진단제공일정적분자병리삼고개치，miR-34c적상조표체여후암전병변음주인소정선성상관，대우지도후암전병변병례적림상관리가능구유중요림상의의。
OBJECTIVE To investigate the expression of miR-34c in laryngeal epitheliual premalignant lesions and to analyze the correlations between its dys-regulation with clinicopathological parameters of laryngeal epitheliual premalignant lesions. METHODS According to 2005 WHO classification system, 94 cases of laryngeal epitheliual premalignant lesion were grouped into mild dysplasia, moderate dysplasia, severe dysplasia and carcinoma in situ. The expression of miR-34c in 94 cases of laryngeal epitheliual premalignant lesion was detected by qRT-PCR, and the correlation of clinicopathological parameters with the clinical and follow-up data of all laryngeal epitheliual premalignant lesions were analyzed. RESULTS The average age of 94 patients with laryngeal epitheliual premalignant lesion was 58 years(ranged from 37 to 86 years). Distribution across the gender was 85 males(90.4%) and 9 females(9.6%). All laryngeal epitheliual premalignant lesions were summarized as follows: 20(21.3%) with mild dysplasia, 23(24.5%) with moderate dysplasia, 26(27.7%) with severe dysplasia, and 25(26.6%) with carcinoma in situ. With the increased grading of dysplasia, miR-34c was up-regulated in laryngeal epitheliual premalignant lesions with significant statistical difference among all groups(F=10.182, P =0.00), While the expression of miR-34c was sharply dropped in saquamous cell carcinoma(SCC) group and even lower than that in normal tissue(NOR) group. On the univariate analysis, miR-34c expression level in laryngeal epitheliual premalignant lesion of alcohol drinking patients was higher than that of non-drinkers. The expression had a significant linear regression relationship with the alcohol drinking(r=0.31, P=0.03). CONCLUSION Up regulation of miR-34c may be helpful for grading of laryngeal epitheliual <br> premalignant lesions and diagnosis of early laryngeal cancer as a valuable molecular markers, and its linear correlation with alcohol drinking of laryngeal epitheliual premalignant lesions showed important guidance implication in clinical <br> management of laryngeal epitheliual premalignant lesions.