CAJ | 학술논문

子宫内膜异位症/病理学%肥大细胞/药物作用%色甘酸钠/药理学%色甘酸钠 /投药和剂量%细胞脱颗粒%肿瘤坏死因子α%类胰蛋白酶类%疾病模型,动物子宮內膜異位癥/病理學%肥大細胞/藥物作用%色甘痠鈉/藥理學%色甘痠鈉 /投藥和劑量%細胞脫顆粒%腫瘤壞死因子α%類胰蛋白酶類%疾病模型,動物
자궁내막이위증/병이학%비대세포/약물작용%색감산납/약이학%색감산납 /투약화제량%세포탈과립%종류배사인자α%류이단백매류%질병모형,동물
Endometriosis/pathology%Mast cells/drug effects%Cromolyn sodium /pharmacology%Cromolyn sodium /administration & dosage%Cell degranulation%Tumor necrosis factor-alpha%Tryptases%Disease models,animal

目的：探讨色甘酸钠干预对子宫内膜异位症大鼠肥大细胞的影响及相关机制。方法：取健康雌性未孕SD大鼠36只，采用自体子宫内膜移植法建立大鼠腹壁子宫内膜异位症模型，2周后根据每天腹腔注射不同剂量色甘酸钠将大鼠分为4组：大剂量组（色甘酸钠20 mg／kg ）、小剂量组（色甘酸钠10 mg／kg ）、阴性对照组（仅注射生理盐水）、空白对照组（模型不作任何处理）。两周后处死大鼠，并收集血液和病灶组织标本，测量各组子宫内膜异位症病灶的大小，并对病灶组织进行苏木素—伊红（ HE）染色观察组织形态及角蛋白和波形蛋白免疫组织化学染色以鉴定造模是否成功。用甲苯胺蓝染色法检测各组病灶肥大细胞总数及其脱颗粒肥大细胞数、酶联免疫吸附试验测定血清肿瘤坏死因子α（ TNF－α）水平、酶免疫分析法测定血清雌二醇水平、免疫组织化学染色法检测子宫内膜异位症病灶肥大细胞中类胰蛋白酶、神经生长因子的表达水平。结果：大剂量组活化的肥大细胞数少于阴性对照组（ P＜0．05），脱颗粒肥大细胞数／肥大细胞总数比值大剂量组小于阴性对照组和空白对照组（均P＜0．05）。同时，该组血清TNF－α水平、病灶类胰蛋白酶表达均小于阴性对照组和空白对照组（均P＜0．05）；但经色甘酸钠治疗后子宫内膜异位症病灶神经生长因子表达差异并无统计学意义（均P＞0．05）。结论：色甘酸钠可能通过稳定肥大细胞抑制其脱颗粒，减少TNF－α、类胰蛋白酶的释放，改善子宫内膜异位症。
목적：탐토색감산납간예대자궁내막이위증대서비대세포적영향급상관궤제。방법：취건강자성미잉SD대서36지，채용자체자궁내막이식법건립대서복벽자궁내막이위증모형，2주후근거매천복강주사불동제량색감산납장대서분위4조：대제량조（색감산납20 mg／kg ）、소제량조（색감산납10 mg／kg ）、음성대조조（부주사생리염수）、공백대조조（모형불작임하처리）。량주후처사대서，병수집혈액화병조조직표본，측량각조자궁내막이위증병조적대소，병대병조조직진행소목소—이홍（ HE）염색관찰조직형태급각단백화파형단백면역조직화학염색이감정조모시부성공。용갑분알람염색법검측각조병조비대세포총수급기탈과립비대세포수、매련면역흡부시험측정혈청종류배사인자α（ TNF－α）수평、매면역분석법측정혈청자이순수평、면역조직화학염색법검측자궁내막이위증병조비대세포중류이단백매、신경생장인자적표체수평。결과：대제량조활화적비대세포수소우음성대조조（ P＜0．05），탈과립비대세포수／비대세포총수비치대제량조소우음성대조조화공백대조조（균P＜0．05）。동시，해조혈청TNF－α수평、병조류이단백매표체균소우음성대조조화공백대조조（균P＜0．05）；단경색감산납치료후자궁내막이위증병조신경생장인자표체차이병무통계학의의（균P＞0．05）。결론：색감산납가능통과은정비대세포억제기탈과립，감소TNF－α、류이단백매적석방，개선자궁내막이위증。
Objective: To investigate the effect of sodium cromoglycate on experimental endometriosis in rats .Methods: Endometriosis model was established in 36 unpregnant female SD rats by transplanting autologous fragments of endometrium to the inner surface of the abdominal wall .The endometriotic lesions were measured by a second laparotomy 2 weeks after surgery .Then the rats were randomly divided into four groups ( n=8 in each group ) to receive intraperitoneal injection of different doses of sodium cromoglycate for 2 weeks: high-dose group ( 20 mg? kg -1? d-1 ); low-dose group(10 mg? kg -1? d-1 ); the negative control group and the blank control group . The animals were sacrificed and the size of the lesions were measured . The endometriosis model of SD rats was identified by HE staining and immunohistochemical staining of keratin and vimentin .The total number of mast cells and their degranulation were measured by Toluidine blue staining; the concentrations of TNF-αin serum were measured by enzyme linked immunosorbent assay; the concentrations of estradiol in serum were measured by enzyme immunoassay; the expression of tryptase and nerve growth factor ( NGF ) were measured by immunohistochemical staining .Results: The number of activated mast cells ( MC) by Toluidine blue staining in high-dose group was significantly lower than that in negative control group ( P <0.05 ), and its ratio of degranulation/total number of MC was significantly lower than that in negative control group or blank control group ( P <0.05 ) .The serum TNF-αlevels and tryptase expression in tissues in high-dose group were significantly lower than those in negative control group or blank control group (P<0.05).However, no significant difference in the size of endometriotic lesions and expression of NGF was found among groups ( P>0 .05 ) . Conclusion: Sodium cromoglycate can stabilize mast cells from degranulation , which may relieve the clinical symptoms of endometriosis by reducing TNF-αand tryptase levels .