CAJ | 학술논문

目的：检测骨巨细胞瘤患者肿瘤组织中S100A8、S100A9mRNA及蛋白的表达水平，探讨其与骨巨细胞瘤的影像学及生物学行为之间的关系。方法：收集2009年1月至2012年6月在上海交通大学医学院附属瑞金医院诊治的43例骨巨细胞瘤患者的临床资料，并采集骨巨细胞瘤新鲜组织和6例正常成人股骨的骨髓组织，运用半定量逆转录PCR及蛋白质印迹法检测肿瘤组织中S100 A8、S100 A9 mRNA及其蛋白表达水平，并分析与不同肿瘤影像学表现如CT检查显示的骨质破坏程度，MRI检查显示的实性成分比例及周边水肿程度等关系。结果：43份骨巨细胞瘤组织中有40份表达S100 A8、S100 A9 mRNA及其蛋白，与正常骨髓组织比较，骨巨细胞瘤组织S100 A8、S100 A9 mRNA及其蛋白表达增加，差异均有统计学意义（均P＜0．05）。影像学不同实质成分比例的骨巨细胞瘤患者S100 A8蛋白表达差异具有统计学意义（ P ＜0．05）；不同骨质破坏程度的骨巨细胞瘤患者S100 A9蛋白表达差异也有统计学意义（ P ＜0．05）。结论：骨巨细胞瘤组织中S100 A8和S100 A9表达增加，并与骨巨细胞瘤骨质破坏程度及实性成分比例、水肿程度具有一定相关性，S100 A8、S100 A9可能参与骨巨细胞瘤的发生发展。
목적：검측골거세포류환자종류조직중S100A8、S100A9mRNA급단백적표체수평，탐토기여골거세포류적영상학급생물학행위지간적관계。방법：수집2009년1월지2012년6월재상해교통대학의학원부속서금의원진치적43례골거세포류환자적림상자료，병채집골거세포류신선조직화6례정상성인고골적골수조직，운용반정량역전록PCR급단백질인적법검측종류조직중S100 A8、S100 A9 mRNA급기단백표체수평，병분석여불동종류영상학표현여CT검사현시적골질파배정도，MRI검사현시적실성성분비례급주변수종정도등관계。결과：43빈골거세포류조직중유40빈표체S100 A8、S100 A9 mRNA급기단백，여정상골수조직비교，골거세포류조직S100 A8、S100 A9 mRNA급기단백표체증가，차이균유통계학의의（균P＜0．05）。영상학불동실질성분비례적골거세포류환자S100 A8단백표체차이구유통계학의의（ P ＜0．05）；불동골질파배정도적골거세포류환자S100 A9단백표체차이야유통계학의의（ P ＜0．05）。결론：골거세포류조직중S100 A8화S100 A9표체증가，병여골거세포류골질파배정도급실성성분비례、수종정도구유일정상관성，S100 A8、S100 A9가능삼여골거세포류적발생발전。
Objective: To investigate the mRNA and protein expression levels of S100A8 and S100A9 in giant cell tumor ( GCT ) of bone, and its relation with radiological findings and biological behavior .Methods:Forty three patient with GCT of bone admitted in Ruijin Hospital Shanghai Jiaotong University School of Medicine from January 2009 to June 2012 were enrolled in the study .The expression levels of S 100 A8 and S100 A9 mRNA and protein were detected by using semiquantitative RT-PCR and Western blotting in 43 specimens of GCT and 6 specimens of normal bone marrow .The CT and MRI findings of patients were retrospectively reviewed , its relation with tissue expression of S100 A8 and S100 A9 was analyzed .Results: Among 43 GCT cases 40 showed positive expression of S 100 A8 and S100 A9 mRNA and protein , and the expression levels were significantly higher than those in normal bone marrow (P<0.05).The expression level of S100A8 protein was significantly different in bone GCT with different composition ratio on MRI ( P <0 .05 ) .The expression level of S100 A9 protein was significantly different in GCT with different degree of bone destruction on CT scan ( P <0.05 ).Conclusion: The expression of S100A8 and S100A9 mRNA and protein is up-regulated in GCT of bone .The expression of S100A8 and S100 A9 is associated with the real composition ratio and the degree of bone destruction, respectively, indicating that S100A8 and S100A9 may be involved in the biological behavior of bone GCT .