CAJ | 학술논문

以白桦（ Betula platyphylla Suks．）木质部为试验材料，分别采用TCA—丙酮提取、酚提取和试剂盒提取3种方法提取白桦木质部总蛋白。利用试剂盒定量和聚丙烯酰胺凝胶电泳、双向电泳技术比较3种提取方法的蛋白质提取效率、提取质量。同时对酚提取法进行改良，建立适用于白桦木质部蛋白的提取方法。研究结果表明，改良的酚提取法提取的白桦木质部蛋白提取量最大，电泳条带清晰，丰富，质量较高。2－DE电泳得到的蛋白凝胶背景干净，蛋白质点清晰。因此，改良的酚提取法是较适合于白桦木质部蛋白的提取方法。
이백화（ Betula platyphylla Suks．）목질부위시험재료，분별채용TCA—병동제취、분제취화시제합제취3충방법제취백화목질부총단백。이용시제합정량화취병희선알응효전영、쌍향전영기술비교3충제취방법적단백질제취효솔、제취질량。동시대분제취법진행개량，건립괄용우백화목질부단백적제취방법。연구결과표명，개량적분제취법제취적백화목질부단백제취량최대，전영조대청석，봉부，질량교고。2－DE전영득도적단백응효배경간정，단백질점청석。인차，개량적분제취법시교괄합우백화목질부단백적제취방법。
With Betula platyphylla Suks xylem, we used TCA-acetone, phenol method, extraction kit methods to extract xylem total protein, and quantitative kit, polyacrylamide gel electrophoresis and two-dimensional electrophoresis for protein ex-traction rate, and extraction quality.We modified phenol extraction method and established suitable method for extraction birch xylem proteins.The protein concentration and quality of phenol extraction was largest.The protein bands were clear and abundant.The gel background was clean, and the protein was clear in 2-DE electrophoresis.Therefore, the modified phenol protocol was more suitable for the protein extraction of Betula platyphylla Suks xylem.