东北林业大学学报
東北林業大學學報
동북임업대학학보
JOURNAL OF NORTHEAST FORESTRY UNIVERSITY
2015年
7期
140-142
,共3页
马尾松%内生真菌%真菌多样性
馬尾鬆%內生真菌%真菌多樣性
마미송%내생진균%진균다양성
Pinus massoniana%Endophytic fungus%Fungi diversity
采用组织块法从马尾松( Pinus massoniana)根、茎和叶中共分离出102株不同形态型的内生真菌,经显微形态观察,将其鉴定为11个属,分属于6科6目4纲3门。其中根部12株涉及6个属,茎部23株涉及9个属,叶部67株涉及11个属。毛霉属( Mucor)和木霉属( Trichoderma)为马尾松内生真菌中的优势属。马尾松内生真菌广泛存在于植株的根、茎、叶中,具有丰富的生物多样性;在不同器官上的种群和数量分布存有明显差异性,从叶部分离到的内生真菌数量和涉及属的数目最多,茎部次之,根部最少。
採用組織塊法從馬尾鬆( Pinus massoniana)根、莖和葉中共分離齣102株不同形態型的內生真菌,經顯微形態觀察,將其鑒定為11箇屬,分屬于6科6目4綱3門。其中根部12株涉及6箇屬,莖部23株涉及9箇屬,葉部67株涉及11箇屬。毛黴屬( Mucor)和木黴屬( Trichoderma)為馬尾鬆內生真菌中的優勢屬。馬尾鬆內生真菌廣汎存在于植株的根、莖、葉中,具有豐富的生物多樣性;在不同器官上的種群和數量分佈存有明顯差異性,從葉部分離到的內生真菌數量和涉及屬的數目最多,莖部次之,根部最少。
채용조직괴법종마미송( Pinus massoniana)근、경화협중공분리출102주불동형태형적내생진균,경현미형태관찰,장기감정위11개속,분속우6과6목4강3문。기중근부12주섭급6개속,경부23주섭급9개속,협부67주섭급11개속。모매속( Mucor)화목매속( Trichoderma)위마미송내생진균중적우세속。마미송내생진균엄범존재우식주적근、경、협중,구유봉부적생물다양성;재불동기관상적충군화수량분포존유명현차이성,종협부분리도적내생진균수량화섭급속적수목최다,경부차지,근부최소。
By means of tissue block method, one hundred and two strains of endophytic fungi were isolated from roots, stems and leaves of Pinus massoniana.They belonged to 11 genera, 6 families, 6 orders, 3 classes, 3 phyla, by morphological iden-tification.Twelve strains of six genera were isolated from roots, 23 strains of 9 genera from stems, and 67 strains of 11 gen-era from leaves.Mucor and Tir chodre ma were the dominant species.The endophytic fungi distributed in roots, stems and leaves of Pinus msa soniana, and the species had broad biodiversity.The quantity and species of the endophytic fungi had great difference among different organs of Pinus massoin ana, with leaves ranking the first, followed by stems and roots.