CAJ | 학술논문

目的：观察两种方式注射白消安对SD大鼠生精功能的影响，探讨以其建立SD大鼠生精功能障碍模型的可行性。方法：观察腹腔注射白消安（分别给予15、20、30、40 mg／kg白消安）与睾丸内注射白消安（分别给予0．1、0．2、0．3和0．4 mg／kg白消安）对SD大鼠存活率、血常规以及睾丸病理Johnsen评分的影响，均设空白对照组、溶剂对照组和阴性对照组。结果：各腹腔注射组大鼠均在30 d内全部死亡，死亡前均表现为虹膜变灰色、眼睑结痂、活动迟缓，另有部分大鼠出现鼻周肛周血迹，血常规检测发现大鼠红细胞、血红蛋白和血小板较空白对照组降低（P＜0．05），睾丸病理Johnsen评分与空白对照组比较，差异无统计学意义（P＞0．05）。各睾丸内注射组大鼠观察30 d内无死亡，血常规检测结果显示大鼠红细胞、血红蛋白及血小板与正常对照组比较，差异无统计学意义（P＞0．05），睾丸病理Johnsen评分均低于正常对照组（ P＜0．05）。结论：腹腔注射白消安，SD大鼠死亡率极高，而睾丸内注射白消安未发现对大鼠有明显毒害作用且0．1、0．2、0．3及0．4 mg／kg的剂量均可建立SD大鼠生精功能障碍模型。
목적：관찰량충방식주사백소안대SD대서생정공능적영향，탐토이기건립SD대서생정공능장애모형적가행성。방법：관찰복강주사백소안（분별급여15、20、30、40 mg／kg백소안）여고환내주사백소안（분별급여0．1、0．2、0．3화0．4 mg／kg백소안）대SD대서존활솔、혈상규이급고환병리Johnsen평분적영향，균설공백대조조、용제대조조화음성대조조。결과：각복강주사조대서균재30 d내전부사망，사망전균표현위홍막변회색、안검결가、활동지완，령유부분대서출현비주항주혈적，혈상규검측발현대서홍세포、혈홍단백화혈소판교공백대조조강저（P＜0．05），고환병리Johnsen평분여공백대조조비교，차이무통계학의의（P＞0．05）。각고환내주사조대서관찰30 d내무사망，혈상규검측결과현시대서홍세포、혈홍단백급혈소판여정상대조조비교，차이무통계학의의（P＞0．05），고환병리Johnsen평분균저우정상대조조（ P＜0．05）。결론：복강주사백소안，SD대서사망솔겁고，이고환내주사백소안미발현대대서유명현독해작용차0．1、0．2、0．3급0．4 mg／kg적제량균가건립SD대서생정공능장애모형。
Aim:To observe the effect of two methods of injecting busulfan to SD rats , and analyze the feasibility of establishing SD rat model of spermatogenesis dysfunction .Methods:Intraperitoneal injection of busulfan (15,20,30 and 40 mg/kg) and testicular injection of busulfan (0.1,0.2,0.3,and 0.4 mg/kg) were performed in the experiment , and surviv-al rate, routine blood test and testicular pathology analysis were compared between them .The rats not treated ( blank con-trol) or given solvent or double distilled water were used as control groups .Results:Ⅰ) SD rats that were given busulfan by intraperitoneal injection all died within 30 d;before death the rats showed dimmed iris , eyelid crusting , slow activity and so on, and some rats showed bleeding around nose and anus .Blood analysis showed that red blood cells , hemoglobin and platelets of the groups given busulfan at different doses decreased significantly compared with blank control group ( P<0.05).Johnsen score had no significant difference between the groups given busulfan at different doses and blank control group(P>0.05).Ⅱ)SD rats that were given busulfan by testicular injection were all alive within 30 days.Blood analysis revealed that red blood cells , hemoglobin and platelets of the groups given busulfan at different doses had no significant difference from those of blank control group (P>0.05),while Johnsen score of the groups given busulfan at different doses was significantly lower than that of blank control group (P<0.05).Conclusion:Intraperitoneal injection of busulfan in rats results in a high mortality.Testicular injection of busulfan(0.1~0.4 mg/kg) in rats showed no significant toxic effects and could be used to establish the model of spermatogenesis dysfunction .