CAJ | 학술논문

目的：研究结直肠癌组织中miRNA表达谱的差异及其相关信号通路。方法：采用SOLiD高通量测序技术对10例结直肠癌组织和10例癌旁正常组织进行测序，然后利用生物信息学方法分析miRNA-mRNA相互作用的通路。结果：筛选出194个结直肠癌差异表达的miRNA，这些miRNA广泛参与基因表达的转录后调控，如MAPK、WNT、TGF-β信号通路。结论：结直肠癌miRNA的差异表达可能暗示着有不同功能或来源的miRNA通过调控不同的靶基因来协同、精细调控某些特定的信号通路。
목적：연구결직장암조직중miRNA표체보적차이급기상관신호통로。방법：채용SOLiD고통량측서기술대10례결직장암조직화10례암방정상조직진행측서，연후이용생물신식학방법분석miRNA-mRNA상호작용적통로。결과：사선출194개결직장암차이표체적miRNA，저사miRNA엄범삼여기인표체적전록후조공，여MAPK、WNT、TGF-β신호통로。결론：결직장암miRNA적차이표체가능암시착유불동공능혹래원적miRNA통과조공불동적파기인래협동、정세조공모사특정적신호통로。
Aim:To investigate the differences of miRNA expression profile and related signaling pathways in colorec -tal cancer .Methods:SOLiD high-throughput sequencing technology was used for investigating differentially expressed miR -NA in the 10 paired colorectal samples(10 colorectal samples and 10 tumor adjacent normal samples ), then, bioinformatic technique was used to construct the miRNA-mRNA interaction pathways of these miRNA using the differentially expressed miRNA.Results:A total of 194 differentially expressed miRNA were obtained , and the screened miRNA were involved in extensive post-transcriptional regulations of gene expression such as MAPK ,WNT,TGF-βsignaling pathway .Conclusion:The differentially expressed miRNA in colorectal cancer tissue may indicate that the miRNA with different functions or from different sources could regulate a certain of signaling pathways synergistically and finely by regulating different target genes .