CAJ | 학술논문

利用AFLP分子标记技术，以部分苹果栽培种和苹果属野生种为参照，对23种红果肉海棠的亲缘关系进行了研究。在64对引物中筛选出4对进行选择性扩增，共得到94条有效谱带，其中多态谱带92条，多态谱带占97．9％。 UPGMA聚类分析显示，30份供试材料的相似系数为0．63～0．95，在相似系数为0．65时，样品可划分为4组，A组中包括6种欧美海棠高原之火、路易莎、秀场、道格、钻石、红巴伦以及粉女士；B组中包括霍巴、丽丝、雷蒙、玛瑙、王族、粉芽、红丽、火焰、完美紫叶、印第安夏天等红果肉杂交种以及野生种新疆红肉苹果、花红和山荆子；C组包括印第安魔力、朱砂、三叶海棠、珠眉海棠、绚丽、凯尔斯、撒氏；D组包括新疆野苹果、富士苹果、嘎啦苹果。从分子水平揭示了它们之间的亲缘关系，为红果肉苹果杂交育种的亲本选配提供了理论依据。
이용AFLP분자표기기술，이부분평과재배충화평과속야생충위삼조，대23충홍과육해당적친연관계진행료연구。재64대인물중사선출4대진행선택성확증，공득도94조유효보대，기중다태보대92조，다태보대점97．9％。 UPGMA취류분석현시，30빈공시재료적상사계수위0．63～0．95，재상사계수위0．65시，양품가화분위4조，A조중포괄6충구미해당고원지화、로역사、수장、도격、찬석、홍파륜이급분녀사；B조중포괄곽파、려사、뢰몽、마노、왕족、분아、홍려、화염、완미자협、인제안하천등홍과육잡교충이급야생충신강홍육평과、화홍화산형자；C조포괄인제안마력、주사、삼협해당、주미해당、현려、개이사、살씨；D조포괄신강야평과、부사평과、알랍평과。종분자수평게시료타문지간적친연관계，위홍과육평과잡교육충적친본선배제공료이론의거。
As a reference to part cultivars and wild crabapple species, amplified fragment length polymorphism (AFLP) analysis was used to reveal the relationships of red-flashed crabapples.Four pairs of primer combinations selected from 64 combina-tions produced 94 legible loci with 92 polymorphic.The polymorphism rate was 97.9%.By UPGMA clustering analysis, the genetic similarity coefficient among 30 samples varied in 0.63-0.95.Four groups of taxa were assigned with the genetic similarity coefficient at 0.65.Group A contained M.‘Prairifire’ , M.‘Louisa’ , M.‘showtime’ , M.‘Dolgo’ , M.‘Spar-kler’, M.‘Red Barron’and M.×domestica‘Pink Lady’; Group B contained red fleshed hybrid like M.‘Hopa’, M.‘Liset’, M.‘Lemoinei’, M.‘Agate’, M.‘Royalty’, M.‘Pink spires’, M.‘Red Splender’, M.‘Flame’, M.‘Per-fect Purple’, M.‘Indian Summer’ and wild species of M.sieversii.var.‘Niedzwetzkyana’, M.asiatica.and M.bacca-ta.;Group C contained M.‘Indian Magic’, M.‘Cinnabar’, M.sieboldii, M.×zumi, M.‘Radiant’, M.‘Kelsey’ and M.‘Sargentii’;Group D contained M.Sieversii, M.×domestica ‘Fuji’and M.×domestica ‘Gala’.The genetic relation-ship between them could be used for parent selection in red-flashed crabapples breeding.