CAJ | 학술논문

目的了解 miRNA‐101在胃癌组织和细胞中的表达水平，以及对胃癌细胞增殖、迁移、侵袭力和皮下移植瘤的影响。方法采用实时定量 PCR 分析28份胃癌组织与人胃癌细胞系BGC‐823、SGC‐7901、MKN‐45、AGS ，以及人胃黏膜上皮细胞系 GES‐1中 miRNA‐101的表达水平。构建 miRNA‐101重组腺病毒表达载体。应用 M TT 法检测 miRNA‐101对胃癌细胞增殖能力的影响。应用 Millipore Transwell小室法检测胃癌细胞的迁移和侵袭能力。使用 BALB／c 裸鼠建立胃癌皮下移植瘤模型，并比较肿瘤体积。统计学分析采用 t 检验。结果胃癌组织中 miRNA‐101的表达量为0．661±0．396，低于所对应的癌旁组织中的1．128±0．697，差异有统计学意义（t ＝10．091，P ＜0．01）。胃正常上皮细胞GES‐1中的miRNA‐101表达量高于胃癌细胞系 BGC‐823、SGC‐7901、MKN‐45和 AGS 。 miRNA‐101对MKN‐45细胞的增殖能力呈现明显的抑制作用，对胃癌细胞 BGC‐823、SGC‐7901、MKN‐45、AGS 的迁移和侵袭均有抑制作用。选取 MKN‐45细胞系建立裸鼠皮下移植瘤模型后5周，miRNA‐101重组腺病毒（Ad‐miRNA‐101）组肿瘤体积为（333．56±46．71） mm3，小于空腺病毒‐增强绿色荧光蛋白（Ad‐EGFP）组的（806．41±51．83） mm3，差异有统计学意义（t ＝21．431，P ＜0．01）。结论在胃组织和细胞中， miRNA‐101是一个抑制性 miRNA ，其表达水平下调参与了胃癌的发生和发展，有可能成为胃癌生物靶向治疗的新靶点。
목적료해 miRNA‐101재위암조직화세포중적표체수평，이급대위암세포증식、천이、침습력화피하이식류적영향。방법채용실시정량 PCR 분석28빈위암조직여인위암세포계BGC‐823、SGC‐7901、MKN‐45、AGS ，이급인위점막상피세포계 GES‐1중 miRNA‐101적표체수평。구건 miRNA‐101중조선병독표체재체。응용 M TT 법검측 miRNA‐101대위암세포증식능력적영향。응용 Millipore Transwell소실법검측위암세포적천이화침습능력。사용 BALB／c 라서건립위암피하이식류모형，병비교종류체적。통계학분석채용 t 검험。결과위암조직중 miRNA‐101적표체량위0．661±0．396，저우소대응적암방조직중적1．128±0．697，차이유통계학의의（t ＝10．091，P ＜0．01）。위정상상피세포GES‐1중적miRNA‐101표체량고우위암세포계 BGC‐823、SGC‐7901、MKN‐45화 AGS 。 miRNA‐101대MKN‐45세포적증식능력정현명현적억제작용，대위암세포 BGC‐823、SGC‐7901、MKN‐45、AGS 적천이화침습균유억제작용。선취 MKN‐45세포계건립라서피하이식류모형후5주，miRNA‐101중조선병독（Ad‐miRNA‐101）조종류체적위（333．56±46．71） mm3，소우공선병독‐증강록색형광단백（Ad‐EGFP）조적（806．41±51．83） mm3，차이유통계학의의（t ＝21．431，P ＜0．01）。결론재위조직화세포중， miRNA‐101시일개억제성 miRNA ，기표체수평하조삼여료위암적발생화발전，유가능성위위암생물파향치료적신파점。
Objective To investigate the expression of microRNA‐101 (miRNA‐101 ) in human gastric cancer ,and to explore its effects on proliferation ,migration and invasion of gastric cancer cell . Methods The expression of miRNA‐101 in 28 human gastric cancer tissues ,human gastric cell lines BGC‐823 , SGC‐7901 , MKN‐45 , AGS and human normal gastric epithelial cell line GES‐1 were determined by real time polymerase chain reaction (PCR) .Recombinant miRNA‐101 adenovirus vector was constructed . The effects of miRNA‐101 on gastric cancer proliferation was detected with cell proliferation assay .The ability of gastric cancer cell migration and invasion was assessed with Transwell assay .Gastric xenograft cancer model was established in BALB /c nude mice and the tumor size was compared .The t test was used for the statistical analysis .Results The expression of miRNA‐101 in gastric cancer tissues was 0 .661 ± 0 .396 ,which was lower than that of corresponding para carcinoma tissues (1 .128 ± 0 .697) ,and the difference was statistically significant (t = 10 .091 , P < 0 .01) .The expression of miRNA‐101 in normal gastric epithelial cell line GES‐1 was higher than those of gastric cancer cell lines BGC‐823 ,SGC‐7901 , MKN‐45 and AGS . There was significant suppression role of miRNA‐101 on MKN‐45 cells proliferation , and which also had inhibition role on cell migration and invasion of gastric cancer cell lines BGC‐823 ,SGC‐7901 ,MKN‐45 and AGS .At five weeks after MKN‐45 gastric xenograft cancer nude mice model established ,the tumor size of Ad‐miRNA‐101 group ((333 .56 ± 46 .71) mm3 ) was smaller than that of Ad‐enhanced green fluorescent protein (EGFP) group (806 .41 ± 51 .83) mm3 ,and the difference was statistically significant (t = 21 .431 , P < 0 .01 ) .Conclusion In gastric tissues and cells ,miRNA‐101 is a tumor suppressive miRNA and its downregulated expression involved in the genesis and development of gastric cancer ,which may be a new target of biological target therapy in gastric cancer .