中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2015年
17期
64-67
,共4页
张付美%李磊%牛慧君%杨廷桐
張付美%李磊%牛慧君%楊廷桐
장부미%리뢰%우혜군%양정동
肺肿瘤%癌前病变%凋亡%生存素%白介素-8%原住杂交
肺腫瘤%癌前病變%凋亡%生存素%白介素-8%原住雜交
폐종류%암전병변%조망%생존소%백개소-8%원주잡교
Lung carcinoma%Precancerous lesion%Apoptosis%Survivin mRNA%Interleukin-8%Hybridization in situ
目的 探讨凋亡抑制蛋白Survivin和血清IL-8在肺鳞癌动物模型发生发展各阶段的表达水平及相关性.方法 Wistar大鼠48只,40只行左肺下叶支气管灌注含三甲基胆蒽(MCA)和二乙基亚硝胺(DEN)的碘油溶液0.lml,另8只灌注0.1ml碘油作为对照,分批处死前行心尖穿刺抽血4～6ml,然后摘除灌注部位肺组织,获取肺鳞癌发生过程各阶段标本;采用双抗体夹心免疫酶标法(ELISA)检测48只大鼠IL-8血清含量,原位杂交法检测各阶段病变组织中Survivin mRNA的转录水平并采用半定量法进行评分.结果 本试验共获取支气管黏膜上皮增生18例,不典型增生11例(含鳞状化生7例),原位癌6例,侵袭癌7例,转移癌6例.根据原位杂交评分在鳞癌组织中Survivin mRNA表达高于癌前病变组、增生组及正常组(P＜0.01);在鳞癌组中转移癌Survivin mRNA表达评分为5.00±0.707,非转移癌的评分为3.08±1.382,二者比较差异有统计学意义(P=0.01).在阴性对照组血清IL-8的表达水平为(24.13±0.97) pg/ml,随着癌变进展而逐步上调,与不典型增生、肺鳞癌阶段IL-8的血清水平比较,差异有统计学意义(P=0.018,P＜0.01).在大鼠肺癌组中,发生肿瘤转移组的血清IL-8水平明显高于非转移组(P =0.002).此外,Survivin mRNA表达阳性的肺组织标本中IL-8浓度为45.910±20.232,Survivin mRNA表达阴性的肺组织标本中IL-8浓度为31.214±11.786,差异有统计学意义(P＜0.01).结论 Survivin和IL-8在大鼠肺鳞癌发生中发挥重要作用,并且协同促进肿瘤的浸润和转移.联合检测可能对肺癌的早期诊断、预后评估有着一定的临床价值.
目的 探討凋亡抑製蛋白Survivin和血清IL-8在肺鱗癌動物模型髮生髮展各階段的錶達水平及相關性.方法 Wistar大鼠48隻,40隻行左肺下葉支氣管灌註含三甲基膽蒽(MCA)和二乙基亞硝胺(DEN)的碘油溶液0.lml,另8隻灌註0.1ml碘油作為對照,分批處死前行心尖穿刺抽血4～6ml,然後摘除灌註部位肺組織,穫取肺鱗癌髮生過程各階段標本;採用雙抗體夾心免疫酶標法(ELISA)檢測48隻大鼠IL-8血清含量,原位雜交法檢測各階段病變組織中Survivin mRNA的轉錄水平併採用半定量法進行評分.結果 本試驗共穫取支氣管黏膜上皮增生18例,不典型增生11例(含鱗狀化生7例),原位癌6例,侵襲癌7例,轉移癌6例.根據原位雜交評分在鱗癌組織中Survivin mRNA錶達高于癌前病變組、增生組及正常組(P＜0.01);在鱗癌組中轉移癌Survivin mRNA錶達評分為5.00±0.707,非轉移癌的評分為3.08±1.382,二者比較差異有統計學意義(P=0.01).在陰性對照組血清IL-8的錶達水平為(24.13±0.97) pg/ml,隨著癌變進展而逐步上調,與不典型增生、肺鱗癌階段IL-8的血清水平比較,差異有統計學意義(P=0.018,P＜0.01).在大鼠肺癌組中,髮生腫瘤轉移組的血清IL-8水平明顯高于非轉移組(P =0.002).此外,Survivin mRNA錶達暘性的肺組織標本中IL-8濃度為45.910±20.232,Survivin mRNA錶達陰性的肺組織標本中IL-8濃度為31.214±11.786,差異有統計學意義(P＜0.01).結論 Survivin和IL-8在大鼠肺鱗癌髮生中髮揮重要作用,併且協同促進腫瘤的浸潤和轉移.聯閤檢測可能對肺癌的早期診斷、預後評估有著一定的臨床價值.
목적 탐토조망억제단백Survivin화혈청IL-8재폐린암동물모형발생발전각계단적표체수평급상관성.방법 Wistar대서48지,40지행좌폐하협지기관관주함삼갑기담은(MCA)화이을기아초알(DEN)적전유용액0.lml,령8지관주0.1ml전유작위대조,분비처사전행심첨천자추혈4～6ml,연후적제관주부위폐조직,획취폐린암발생과정각계단표본;채용쌍항체협심면역매표법(ELISA)검측48지대서IL-8혈청함량,원위잡교법검측각계단병변조직중Survivin mRNA적전록수평병채용반정량법진행평분.결과 본시험공획취지기관점막상피증생18례,불전형증생11례(함린상화생7례),원위암6례,침습암7례,전이암6례.근거원위잡교평분재린암조직중Survivin mRNA표체고우암전병변조、증생조급정상조(P＜0.01);재린암조중전이암Survivin mRNA표체평분위5.00±0.707,비전이암적평분위3.08±1.382,이자비교차이유통계학의의(P=0.01).재음성대조조혈청IL-8적표체수평위(24.13±0.97) pg/ml,수착암변진전이축보상조,여불전형증생、폐린암계단IL-8적혈청수평비교,차이유통계학의의(P=0.018,P＜0.01).재대서폐암조중,발생종류전이조적혈청IL-8수평명현고우비전이조(P =0.002).차외,Survivin mRNA표체양성적폐조직표본중IL-8농도위45.910±20.232,Survivin mRNA표체음성적폐조직표본중IL-8농도위31.214±11.786,차이유통계학의의(P＜0.01).결론 Survivin화IL-8재대서폐린암발생중발휘중요작용,병차협동촉진종류적침윤화전이.연합검측가능대폐암적조기진단、예후평고유착일정적림상개치.
Objective To investigate the expression levels of Survivin mRNA and the serum interleukin-8 in the rat lung squamous carcinoma and their correlation.Methods Forty-eight wistar rats were instilled with 3 Methylcholanthrene (MCA) and Diethylinitrosamine(DEN) into the left lobarbranchus to induce lung squamous cell carcinoma,the other 8 rats instilled with iodized oil were regarded as control group.Specimens were obtained in every pathological phase during the carcinogenesis,these rats were at different intervals.The expression levels of Survivin mRNA in each patholonical phases during the carcinoma were examined by hybridization in situ.The score of hybridization in situ was calculated by combining an estimate of the percentage of immunoreactive cells with that of the stain intensity.The serum level of IL-8 was measured in 48 rats with pathological phase and healthy control by enzyme-linked immunosorbent assay (ELISA).Results Forty-right specimens of every patholonical phase during the carcinonenesis showed:18 cases of hyperplasia,11 cases of dysplasia (including 7 cases of squamous metaplasia),6 cases of carcinoma in situ,7 cases of infiltrating camcinoma and 6 cases of metastasis.For the rat bronchial epithelium cells in control group,hyperplasia,precancerous lesions,and lung squamous cell carcinoma,the sorce of Survivin mRNA in lung tissue was significantly increased in hyperplasia,precancerous lesion and lung squamous carcinogenesis (P ＜ 0.001).In squamous carcinogenesis group,the sorce of Survivin mRNA in metastatic carcinoma and non-metastasis was 5.00 ± 0.707,3.08 ± 1.382,respectively.The difference was significant(P =0.01).The serum IL-8 level of the healthy control was (24.13 ± 0.97)pg/ml,the serum IL-8 level was significantly increased in dysplasia and carcinoma(P =0.018,P ＜ 0.001,respectively).In squamous carcinogenesis group,the serum IL-8 level in metastasis was higher than that of non metastasis (P =0.002).In addition,there was a positive relationship between the expression of Survivin and IL-8.The serum level of IL-8 in cases of Survivinpostive group was 45.910 ± 20.232,which was significantly higher than that in cases of Survivin-negative group (31.214 ± 11.786),there was significant difference between the two groups(P ＜0.01).Conclusions Survivin and IL-8 play important roles in the carcinonenesis of inducing rat lung squamous cell carcinoma by MCA and DEN.Survivin in coordination with IL-8 to promote neoplastic infiltration and metastasis.Combined detection of IL-8 and Survivin is of significance in diagnosing and evaluating the prognosis,and it is worthy of futher study.
