中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2015年
8期
753-757
,共5页
陈卫良%杨理坤%匡红%陈磊%王玉海
陳衛良%楊理坤%劻紅%陳磊%王玉海
진위량%양리곤%광홍%진뢰%왕옥해
脑水肿%颅内压%动物模型
腦水腫%顱內壓%動物模型
뇌수종%로내압%동물모형
Brain edema%Intracranial pressure%Models,animal
目的 利用窦汇区球囊加压法建立兔外伤性急性弥漫性脑肿胀(PADBS)模型,为探讨PADBS的发生机制提供依据. 方法 新西兰兔50只按随机数字表法分为对照组(10只)和模型组(40只).使用颅内压探头,制备窦汇区球囊压迫模型,模型组又分为加压1.5h组、解压后1.5h组、解压后3h组和解压后4.5h组4个亚组(每组各10只),动态监测各组颅内压、脑含水量、病理及超微结构等变化. 结果 加压1.5h组死亡1只,解压后1.5h组死亡1只,解压后3h组死亡2只,解压后4.5h组死亡3只,模型制备成功率为83％ (33/40).对照组、加压1.5h组、解压后1.5h组、解压后3h组、解压后4.5h组颅内压分别为(4.9 ±0.8)mmHg、(50.1 ±4.3)mmHg、(45.2±1.7)mmHg、(48.6±2.2) mmHg、(59.1±2.5) mmHg(P ＜0.05).对照组、加压1.5h组、解压后1.5h组、解压后3h组、解压后4.5h组脑含水量分别为(75.0±0.6)％、(76.7±0.8)％、(77.3±0.5)％、(78.5±0.6)％、(79.4±0.7)％(P＜0.05).解压后1.5h组出现血管源性脑肿胀;解压后3h组脑组织细胞破坏明显,细胞毒性脑肿胀形成;解压后4.5h组高颅压和细胞缺血缺氧的恶性循环形成. 结论 颅内压探头监测可保证稳定的球囊压力,建立的PADBS动物模型具有良好的可靠性和稳定性,为进一步探索PADBS的可能机制和治疗策略提供依据.
目的 利用竇彙區毬囊加壓法建立兔外傷性急性瀰漫性腦腫脹(PADBS)模型,為探討PADBS的髮生機製提供依據. 方法 新西蘭兔50隻按隨機數字錶法分為對照組(10隻)和模型組(40隻).使用顱內壓探頭,製備竇彙區毬囊壓迫模型,模型組又分為加壓1.5h組、解壓後1.5h組、解壓後3h組和解壓後4.5h組4箇亞組(每組各10隻),動態鑑測各組顱內壓、腦含水量、病理及超微結構等變化. 結果 加壓1.5h組死亡1隻,解壓後1.5h組死亡1隻,解壓後3h組死亡2隻,解壓後4.5h組死亡3隻,模型製備成功率為83％ (33/40).對照組、加壓1.5h組、解壓後1.5h組、解壓後3h組、解壓後4.5h組顱內壓分彆為(4.9 ±0.8)mmHg、(50.1 ±4.3)mmHg、(45.2±1.7)mmHg、(48.6±2.2) mmHg、(59.1±2.5) mmHg(P ＜0.05).對照組、加壓1.5h組、解壓後1.5h組、解壓後3h組、解壓後4.5h組腦含水量分彆為(75.0±0.6)％、(76.7±0.8)％、(77.3±0.5)％、(78.5±0.6)％、(79.4±0.7)％(P＜0.05).解壓後1.5h組齣現血管源性腦腫脹;解壓後3h組腦組織細胞破壞明顯,細胞毒性腦腫脹形成;解壓後4.5h組高顱壓和細胞缺血缺氧的噁性循環形成. 結論 顱內壓探頭鑑測可保證穩定的毬囊壓力,建立的PADBS動物模型具有良好的可靠性和穩定性,為進一步探索PADBS的可能機製和治療策略提供依據.
목적 이용두회구구낭가압법건립토외상성급성미만성뇌종창(PADBS)모형,위탐토PADBS적발생궤제제공의거. 방법 신서란토50지안수궤수자표법분위대조조(10지)화모형조(40지).사용로내압탐두,제비두회구구낭압박모형,모형조우분위가압1.5h조、해압후1.5h조、해압후3h조화해압후4.5h조4개아조(매조각10지),동태감측각조로내압、뇌함수량、병리급초미결구등변화. 결과 가압1.5h조사망1지,해압후1.5h조사망1지,해압후3h조사망2지,해압후4.5h조사망3지,모형제비성공솔위83％ (33/40).대조조、가압1.5h조、해압후1.5h조、해압후3h조、해압후4.5h조로내압분별위(4.9 ±0.8)mmHg、(50.1 ±4.3)mmHg、(45.2±1.7)mmHg、(48.6±2.2) mmHg、(59.1±2.5) mmHg(P ＜0.05).대조조、가압1.5h조、해압후1.5h조、해압후3h조、해압후4.5h조뇌함수량분별위(75.0±0.6)％、(76.7±0.8)％、(77.3±0.5)％、(78.5±0.6)％、(79.4±0.7)％(P＜0.05).해압후1.5h조출현혈관원성뇌종창;해압후3h조뇌조직세포파배명현,세포독성뇌종창형성;해압후4.5h조고로압화세포결혈결양적악성순배형성. 결론 로내압탐두감측가보증은정적구낭압력,건립적PADBS동물모형구유량호적가고성화은정성,위진일보탐색PADBS적가능궤제화치료책략제공의거.
Objective To establish a rabbit posttraumatic acute diffuse brain swelling (PADBS) model and investigate the mechanism of action.Methods Fifty New Zealand rabbits were assigned to control group (n =10) and model group (n =40) according to random number table.The animal model of sinus balloon compression was established under intracranial pressure monitoring by using intracranial pressure probe.The model group was subdivided equally at 1.5 hours after compression,1.5 hours after decompression,3 hours after decompression and 4.5 hours after decompression,for which intracranial pressure,brain water content,pathological mechanism and ultrastructure were measured dynamically.Results The success rate of modeling was 83％ (33/40).Intracranial pressure was (4.9 ± 0.8)mmHg in control group,(50.1 ± 4.3) mmHg in 1.5 hours after compression group,(45.2 ± 1.7) mmHg in 1.5 hours after decompression group,(48.6 ± 2.2) mmHg in 3 hours after decompression group,and (59.1 ±2.5)mmHg in 4.5 hours after decompression group (P ＜0.05).Brain water content was (75.0 ± 0.6) ％ in control gorup,(76.7 ± 0.8) ％ in 1.5 hours after compression group,(77.3 ± 0.5) ％ in 1.5 hours after decompression group,(78.5 ± 0.6) ％ in 3 hours after decompression group,and (79.4 ± 0.7) ％ in 4.5 hours after decompression group (P ＜ 0.05).Vasogenic brain oedema was seen 1.5 hours after decompression.Cytotoxicity brain swelling generated with brain tissue destroyed 3 hours after decompression.The vicious cycle of high intracranial pressure and brain tissue destruction occurred 4.5 hours after decompression.Conclusion Under intracranial pressure probe monitoring,the rabbit model of PADBS by sinus balloon compression has stable pressure of the sinus balloon and has good reliability and repeatability,which provides a reliable evidence for further study on the possible mechanism and treatment methods of PADBS.
