CAJ | 학술논문

目的：检测结肠癌患者血浆中 microRNA-181b(miR-181b)的水平变化,并初步探讨其临床意义。方法采用茎环定量逆转录聚合酶链反应(RT-PCR)方法检测42例结肠癌患者及42例健康人血浆中 miR-181b 的水平,并分析 miR-181b 与结肠癌临床病理特征之间的关系。结果与正常对照组(0.38±0.13)相比,结肠癌患者血浆中miR-181b 水平(0.64±0.22)明显升高,二者间差异有统计学意义(P <0.01)；结肠癌患者血浆中 miR-181b 水平在不同性别、年龄及肿瘤大小之间差异无统计学意义(P >0.05),而在不同淋巴转移、远处转移及临床分期间的差异具有统计学意义(P <0.05)。结论结肠癌患者血浆中 miR-181b 水平增高,miR-181b 可能参与了结肠癌的发生发展过程。
목적：검측결장암환자혈장중 microRNA-181b(miR-181b)적수평변화,병초보탐토기림상의의。방법채용경배정량역전록취합매련반응(RT-PCR)방법검측42례결장암환자급42례건강인혈장중 miR-181b 적수평,병분석 miR-181b 여결장암림상병리특정지간적관계。결과여정상대조조(0.38±0.13)상비,결장암환자혈장중miR-181b 수평(0.64±0.22)명현승고,이자간차이유통계학의의(P <0.01)；결장암환자혈장중 miR-181b 수평재불동성별、년령급종류대소지간차이무통계학의의(P >0.05),이재불동림파전이、원처전이급림상분기간적차이구유통계학의의(P <0.05)。결론결장암환자혈장중 miR-181b 수평증고,miR-181b 가능삼여료결장암적발생발전과정。
Objective To investigate the plasma level and clinical significance of microRNA-181b (miR-181b)in patients with colon cancer.Methods The plasma level of miR-181b in 42 patients with colon cancer and 42 healthy controls were detected by stem-loop RT-PCR.The relationship between clinicopathological characteristics of colon cancer and miR-181b was analyzed.Results Compared with healthy controls,the plasma miR-181b in patients with colon cancer was significantly higher (0.38±0.13 vs 0.64±0.22,respectively,P <0.01).There was no significant difference between plasma miR-181b and gender,age and tumor size in patients with colon cancer (P >0.05).There were significant differences between plasma miR-181b and lymph node metastasis,distant metastasis and tumor stage (P <0.05).Conclusion The plasma miR-181b in patients with colon cancer increased and miR-181b may contribute to the development of colon cancer.