CAJ | 학술논문

DNA甲基化作为表观遗传修饰的主要途径之一,能够通过对基因组 DNA 的修饰参与植物对外界环境胁迫的响应, DNA甲基化需要DNA甲基转移酶的参与.实验室前期研究表明,茶树在冷驯化过程中发生了甲基化反应.通过RACE克隆,获得了茶树中DNA甲基转移酶CsDRM2基因的cDNA全长序列(NCBI登录号KR057963).CsDRM2序列全长2328bp,含1815bp的开放阅读框,编码604个氨基酸,预测蛋白质分子量为67.39 kD,理论等电点(PI)为4.85.生物信息学分析结果显示,茶树CsDRM2蛋白与芝麻和烟草的亲缘关系最近,CsDRM2的氨基酸序列与其他植物的DRM2相似性均高于65%.CsDRM2基因表达分析的结果发现,随着冷驯化的进行,CsDRM2基因的表达量呈现出增加的趋势,参与茶树冷驯化过程的甲基化响应.
DNA갑기화작위표관유전수식적주요도경지일,능구통과대기인조 DNA 적수식삼여식물대외계배경협박적향응, DNA갑기화수요DNA갑기전이매적삼여.실험실전기연구표명,다수재랭순화과정중발생료갑기화반응.통과RACE극륭,획득료다수중DNA갑기전이매CsDRM2기인적cDNA전장서렬(NCBI등록호KR057963).CsDRM2서렬전장2328bp,함1815bp적개방열독광,편마604개안기산,예측단백질분자량위67.39 kD,이론등전점(PI)위4.85.생물신식학분석결과현시,다수CsDRM2단백여지마화연초적친연관계최근,CsDRM2적안기산서렬여기타식물적DRM2상사성균고우65%.CsDRM2기인표체분석적결과발현,수착랭순화적진행,CsDRM2기인적표체량정현출증가적추세,삼여다수랭순화과정적갑기화향응.
As one of the major pathways of epigenetic regulation, DNA methylation can modify genomic DNA and take part in responding to the environmental stresses of the plants. The process requires the involvement of DNA methyltransferase. It was found, in our previous study, to occur during the cold acclimation. In this study, a full-length cDNA sequence ofCsDRM2 gene was obtained using the RACE technique. Subsequently, it was submitted to NCBI and assigned an accession number of KR057963. The cDNA length ofCsDRM2 was 2,328 bp containing a 1,815 bp open reading frame and the encoding 604 amino acid residues. The predicted molecular weight was 67.39 kD and theoretical isoelectric point pH 4.85. The comparison on their sequences showed a similarity greater than 65% betweenCsDRM2 and other reported DRMs. The phylogenetic tree analysis onCsDRM2 indicated its close genetic relationships withSesamum indicum andNicotiana tabacum. The qRT-PCR results onCsDRM2 revealed differential expressions of the gene at different stages of the cold acclimation. It suggested thatCsDRM2 participated in the DNA methylation of the tea plant in response to cold stress.