CAJ | 학술논문

目的:研究经典古方肝胆两益汤治疗失眠症的作用机制,为临床合理使用肝胆两益汤提供参考.方法:将56只SD大鼠随机分为正常组、模型组、地西泮组(0.001 g·kg-1)、百乐眠组(0.215 g·kg-1)和肝胆两益汤低剂量组(0.925 g·kg-1)、中剂量组(1.850 g·kg-1)和高剂量组(3.700 g·kg-1),采用连续腹腔注射对氯苯丙氨酸(0.3 g·kg-1)造失眠大鼠模型,灌胃肝胆两益汤治疗,运用高效液相-荧光检测器,检测大鼠皮层、海马、纹状体、下丘脑和脑干内5-HT、DA和NE的含量.结果:与正常组相比,模型组大鼠皮层、海马、纹状体、下丘脑和脑干内5-HT含量显著降低(P < 0.01),下丘脑DA含量显著降低(P < 0.05),纹状体和下丘脑NE含量升高(P < 0.05).经灌胃给予肝胆两益汤治疗后大鼠海马、皮层、纹状体和下丘脑内5-HT含量与模型组相比显著提高,下丘脑内NE含量与模型组相比显著降低(P < 0.05),其中最佳剂量为1.850 g·kg-1.结论:肝胆两益汤可调节机体神经内分泌系统,具有显著的镇静催眠和治疗失眠的作用.其对失眠治疗的可能作用机制是通过增加脑中5-HT含量和降低下丘脑内NE含量.
목적:연구경전고방간담량익탕치료실면증적작용궤제,위림상합리사용간담량익탕제공삼고.방법:장56지SD대서수궤분위정상조、모형조、지서반조(0.001 g·kg-1)、백악면조(0.215 g·kg-1)화간담량익탕저제량조(0.925 g·kg-1)、중제량조(1.850 g·kg-1)화고제량조(3.700 g·kg-1),채용련속복강주사대록분병안산(0.3 g·kg-1)조실면대서모형,관위간담량익탕치료,운용고효액상-형광검측기,검측대서피층、해마、문상체、하구뇌화뇌간내5-HT、DA화NE적함량.결과:여정상조상비,모형조대서피층、해마、문상체、하구뇌화뇌간내5-HT함량현저강저(P < 0.01),하구뇌DA함량현저강저(P < 0.05),문상체화하구뇌NE함량승고(P < 0.05).경관위급여간담량익탕치료후대서해마、피층、문상체화하구뇌내5-HT함량여모형조상비현저제고,하구뇌내NE함량여모형조상비현저강저(P < 0.05),기중최가제량위1.850 g·kg-1.결론:간담량익탕가조절궤체신경내분비계통,구유현저적진정최면화치료실면적작용.기대실면치료적가능작용궤제시통과증가뇌중5-HT함량화강저하구뇌내NE함량.
Objective: To study the mechanism of Gandanliangyitang (GDLYT) in the treatment of insomnia and provide reference for clinical use.Methods:A total of 56 SD rats were randomly divided into normal group, model group, diazepam group (0.001 g·kg-1),Bailemian group (0.215 g·kg-1) and GDLYT low dose group (0.925 g·kg-1), middle dose group (1.850 g·kg-1) and high dose group (3.700 g·kg-1). The model of insomnia rats was made successfully by using continuous intraperitoneal injection of parachlorophenylalanine (0.3 g·kg-1). The contents of 5-HT, DA and NE in cortex, hippocampus, corpus striatum, hypothalamus and brainstem of rats were detected by using high performance liquid chromatography with lfuorescence detector.Results: Compared with normal group, in model group, the content of 5-HT in rat cortex and hippocampus, striatum, hypothalamus and brainstem significantly decreased (P < 0.01), DA content in the hypothalamus significantly decreased (P < 0.05), NE content increased in striatum and hypothalamus (P < 0.05). Compared with the model group, in GDLYT treatment group, the content of 5-HT in hippocampus, cortex, striatum and hypothalamus increased signiifcantly, NE content in hypothalamus signiifcantly decreased (P < 0.05). The best dose is 1.850 g·kg-1.Conclusion:GDLYT plays remarkable role on sedative hypnotic, the treatment mechanism of insomnia is probably by increasing the 5-HT content in the brain and decreasing the content of NE in hypothalamus, regulating neuroendocrine system.