中国药物应用与监测
中國藥物應用與鑑測
중국약물응용여감측
CHINESE JOURNAL OF DRUG APPLICATION AND MONITORING
2015年
4期
209-211
,共3页
王天琳%郭代红%柴栋%张波%王睿
王天琳%郭代紅%柴棟%張波%王睿
왕천림%곽대홍%시동%장파%왕예
利奈唑胺%血小板%凋亡%乳酸脱氢酶%Caspase-3
利奈唑胺%血小闆%凋亡%乳痠脫氫酶%Caspase-3
리내서알%혈소판%조망%유산탈경매%Caspase-3
Linezolid%Platelet%Apoptosis%Lactic dehydrogenase%Caspase-3
目的:研究利奈唑胺对人血小板的凋亡作用.方法:用两步离心法制备人富血小板血浆并纯化血小板,用酶联免疫吸附法检测乳酸脱氢酶(LDH)评价血小板损伤程度,用Annexin V法检测磷酯酰丝氨酸外翻,用比色法检测Caspase-3活性.结果:与空白对照组相比,利奈唑胺剂给药4 h可剂量依赖性引起磷酯酰丝氨酸外翻增加,Caspase-3活性增加.150μg·mL-1利奈唑胺给药4 h可引起血小板LDH释放增加.结论:利奈唑胺可引起人血小板凋亡.
目的:研究利奈唑胺對人血小闆的凋亡作用.方法:用兩步離心法製備人富血小闆血漿併純化血小闆,用酶聯免疫吸附法檢測乳痠脫氫酶(LDH)評價血小闆損傷程度,用Annexin V法檢測燐酯酰絲氨痠外翻,用比色法檢測Caspase-3活性.結果:與空白對照組相比,利奈唑胺劑給藥4 h可劑量依賴性引起燐酯酰絲氨痠外翻增加,Caspase-3活性增加.150μg·mL-1利奈唑胺給藥4 h可引起血小闆LDH釋放增加.結論:利奈唑胺可引起人血小闆凋亡.
목적:연구리내서알대인혈소판적조망작용.방법:용량보리심법제비인부혈소판혈장병순화혈소판,용매련면역흡부법검측유산탈경매(LDH)평개혈소판손상정도,용Annexin V법검측린지선사안산외번,용비색법검측Caspase-3활성.결과:여공백대조조상비,리내서알제급약4 h가제량의뢰성인기린지선사안산외번증가,Caspase-3활성증가.150μg·mL-1리내서알급약4 h가인기혈소판LDH석방증가.결론:리내서알가인기인혈소판조망.
Objective: To study the effect of linezolid on the apoptosis of human platelet.Methods:Two-step centrifugation was used for platelet enrichment and puriifcation. LDH concentration in the supernatant was determined by ELISA, phosphatidylserine (PS) exposure by Annexin V-binding, and the activation of Caspase-3 by colorimetry.Results:Four hours exposure to linezolid triggered Annexin V-binding, activated Caspase-3 in dose dependent manner. Linezolid 150 μg·mL-1·4 h-1can stimulate the release of LDH.Conclusion:Linezolid can induce the apoptosis of human platelet.
