中国饲料
中國飼料
중국사료
CHINA FEED
2015年
2期
31-33,37
,共4页
李人宇%彭松%李咏梅%陶明涛
李人宇%彭鬆%李詠梅%陶明濤
리인우%팽송%리영매%도명도
分光光度法%牛磺酸%溴酚蓝%饲料%测定
分光光度法%牛磺痠%溴酚藍%飼料%測定
분광광도법%우광산%추분람%사료%측정
spectrophotometry%taurine method%bromophenol blue%feed%determination
本实验研究了牛磺酸对高锰酸钾氧化溴酚蓝反应的促进作用，建立了分光光度法测定牛磺酸的新方法。结果表明：最大吸收波长为400 nm，牛磺酸浓度为0~0.15μg/mL时符合比耳定律，表观摩尔吸光系数为6.25×105 L/mol·cm，检出限为2.63μg/L。方法用于测定饲料中牛磺酸含量，结果与高效液相色谱法一致，标准偏差为0.85%~1.31%（n=6），回收率为97.0%~101.8%。
本實驗研究瞭牛磺痠對高錳痠鉀氧化溴酚藍反應的促進作用，建立瞭分光光度法測定牛磺痠的新方法。結果錶明：最大吸收波長為400 nm，牛磺痠濃度為0~0.15μg/mL時符閤比耳定律，錶觀摩爾吸光繫數為6.25×105 L/mol·cm，檢齣限為2.63μg/L。方法用于測定飼料中牛磺痠含量，結果與高效液相色譜法一緻，標準偏差為0.85%~1.31%（n=6），迴收率為97.0%~101.8%。
본실험연구료우광산대고맹산갑양화추분람반응적촉진작용，건립료분광광도법측정우광산적신방법。결과표명：최대흡수파장위400 nm，우광산농도위0~0.15μg/mL시부합비이정률，표관마이흡광계수위6.25×105 L/mol·cm，검출한위2.63μg/L。방법용우측정사료중우광산함량，결과여고효액상색보법일치，표준편차위0.85%~1.31%（n=6），회수솔위97.0%~101.8%。
A new spectrophotometric method for determination of taurine was developed after the promotive effect of taurine on the reaction of potassium hypermanganate oxidizing bromophenol blue was studied.The results showed that the maximum absorption wavelength located at 400 nm.The Beer’s law was obeyed in the range of 0~0.15μg/mL for taurine with the apparent molar absorption coefficient of 6.25 ×105 L/(mol·cm)and the detection limit of 2.63 μg/L.This method was applied to the determination of taurine content in feed.The results agreed well with those obtained by high perfor-mance liquid chromatography.The relative standard deviation was 0.85% ~ 1.31%(n=6),and the average recovery was 97.0%~101.8%.
