CAJ | 학술논문

利用Plackett-Burman实验、最陡爬坡实验和中心组合设计对产香叶醇重组大肠杆菌的发酵培养基进行了优化。首先运用Plackett-Burman设计对7种培养基组分进行了筛选；然后运用最陡爬坡法使关键因素的浓度接近最佳响应区域；最后通过中心组合设计确定了关键因素的最佳浓度及回归模型。结果表明柠檬酸铁铵、硫酸镁和微量元素混合液为影响香叶醇产量的关键因素,其最佳浓度分别为0．13 g/L,2.12 g/L和16.7 mL/L,在此条件下香叶醇的产量可达223.24 mg/L,是优化前香叶醇产量(68.5 mg/L)的3．26倍。
이용Plackett-Burman실험、최두파파실험화중심조합설계대산향협순중조대장간균적발효배양기진행료우화。수선운용Plackett-Burman설계대7충배양기조분진행료사선；연후운용최두파파법사관건인소적농도접근최가향응구역；최후통과중심조합설계학정료관건인소적최가농도급회귀모형。결과표명저몽산철안、류산미화미량원소혼합액위영향향협순산량적관건인소,기최가농도분별위0．13 g/L,2.12 g/L화16.7 mL/L,재차조건하향협순적산량가체223.24 mg/L,시우화전향협순산량(68.5 mg/L)적3．26배。
The Plackett-Burman experiment, the steepest ascent experiment and the central composite design were used to optimize the fermentation medium for the production of geraniol by the recombinant E. coli. Firstly, Plackett-Burman design was used to screen the critical factors which influenced the geraniol production from seven mediums. Secondly, the method of steepest ascent was adopted to make the key factors approach the optimal response area. Finally, the optimal concentration of key factors and regression model were obtained by central composite design. It was indicated that ammonium ferric citrate, MgSO4 , and trace element solution were identified to be the significant factors and the it optimal concentrations were 0. 13 g/L, 2. 12 g/L, and 16. 7 mL/L, respectively. The geraniol concentration increased to 223. 24 mg/L under these conditions. It was about 3. 26 times more than that of before.