CAJ | 학술논문

本试验以果胶为唯一碳源设计筛选培养基，从蚕沙堆放地筛选出一株能利用蚕沙发酵产果胶酶的菌株D80，表型分析及ITS rDNA序列分子鉴定结果表明该菌株为棘孢曲霉（Aspergillus aculeatus）。利用微波照射对D80进行诱变育种，获得一株酶活力有大幅度提高的正突变株DW75，在初始发酵条件下测得诱变菌株酶活为10.166 U/mL，是未诱变菌株D80酶活的2.47倍，对菌株DW75进行5次传代培养发酵，测得酶活均较稳定，表明DW75是一株遗传性状稳定的正突变株。
본시험이과효위유일탄원설계사선배양기，종잠사퇴방지사선출일주능이용잠사발효산과효매적균주D80，표형분석급ITS rDNA서렬분자감정결과표명해균주위극포곡매（Aspergillus aculeatus）。이용미파조사대D80진행유변육충，획득일주매활력유대폭도제고적정돌변주DW75，재초시발효조건하측득유변균주매활위10.166 U/mL，시미유변균주D80매활적2.47배，대균주DW75진행5차전대배양발효，측득매활균교은정，표명DW75시일주유전성상은정적정돌변주。
In this experiment,screening medium was designed used pectin as the sole carbon source,to screen a strain named D80 that could produce pectinase by silkworm faeces fermentation.The strain was identified as Aspergillus aculeatus by morphological observation and ITS rDNA homogeneous analysis.Then D80 was treated with microwave irradi-ation mutation,a positive mutant strain DW75 was screened,enzyme activity of which increased tremendously.In the ini-tial fermentation conditions,enzyme activity of the strain DW75 was 10.166 U/mL,147% higher than that of the origina strain.After 5 times of subculture fermentation,the enzyme activity were relatively stable,which showed that DW75 was an excellent genetic stable mutant strain.