CAJ | 학술논문

以1 g油橄榄叶提取物为原料,采用高效液相色谱法测定油橄榄叶橄榄苦苷( OE)和羟基酪醇( HT)的含量,筛选得到适合用于OE酶解制备HT的酶,通过单因素和正交试验优化酶解工艺,并考察了HT、OE和油橄榄叶提取物清除二苯基苦基肼( DPPH)自由基的抗氧化活性。结果表明,在相同酶活力条件下,半纤维素酶对OE的降解和HT的制备都有着很好的效果,其次为纤维素酶和β-葡萄糖苷酶。半纤维素酶降解OE的最佳工艺为温度60℃、pH值5.5、酶量40 mg、时间6 h,得到HT质量分数为6.07%,OE降解率为85.28%。酸解、碱解和酶解方法的比较结果表明,盐酸水解后HT质量分数可达7.41%,效果略优于酶水解；但NaOH水解后HT质量分数仅为4.09%。 DPPH自由基清除实验结果表明,DPPH自由基清除能力为HT>维生素C>OE>2,6-二叔丁基-4-甲基苯酚>油橄榄叶提取物,HT的半数抑制质量浓度(IC50)值可达1.07 mg/L。
이1 g유감람협제취물위원료,채용고효액상색보법측정유감람협감람고감( OE)화간기락순( HT)적함량,사선득도괄합용우OE매해제비HT적매,통과단인소화정교시험우화매해공예,병고찰료HT、OE화유감람협제취물청제이분기고기정( DPPH)자유기적항양화활성。결과표명,재상동매활력조건하,반섬유소매대OE적강해화HT적제비도유착흔호적효과,기차위섬유소매화β-포도당감매。반섬유소매강해OE적최가공예위온도60℃、pH치5.5、매량40 mg、시간6 h,득도HT질량분수위6.07%,OE강해솔위85.28%。산해、감해화매해방법적비교결과표명,염산수해후HT질량분수가체7.41%,효과략우우매수해；단NaOH수해후HT질량분수부위4.09%。 DPPH자유기청제실험결과표명,DPPH자유기청제능력위HT>유생소C>OE>2,6-이숙정기-4-갑기분분>유감람협제취물,HT적반수억제질량농도(IC50)치가체1.07 mg/L。
The hydroxytyrosol ( HT ) was prepared from oleuropein ( OE ) in the olive leaves by enzymatic hydrolysis. The qualitative and quantitative determination of OE and HT from the olive leaf extract were analysed by HPLC, and the best enzyme to hydrolyze OE for HT from olive leaf extrct was selected. Moreover, the optimal technology of enzymatic process was obtained by single factor test and orthogonal test. The DPPH radical scavenging effects of HT, OE and olive leaf extract were examined, too. The results showed that hemicellulase was the best enzyme to hydrolyze OE for HT in the same enzymatic activity from 10 enzymes, and the cellulase and β-glucosidase were second. The optimal conditions were temperature 60 ℃, pH value 5. 5, enzyme quantity 40 mg and time 6 h. The degradation rate of OE was 85. 28 %, and HT content was 6. 07 % by the best enzymatic process. Furthermore, HT was obtained by acid- and base-treatment, respectively. Acid hydrolysis performance (7. 41 %) was better than enzymatic hydrolysis, and base-treatment result(4. 09 %) was the worst. By comparison of the two methods, it was found that enzymatic condition was mild and could reduce the catalyst use, and it is a new hydrolysis method to further develop and utilize. The IC50 of HT was 1. 07 mg/L, and the radical scavenging effect was in the order of HT>Vc>OE>BHT>olive leaf extract. Therefore, HT has a good ability of anti-oxidation compound.