CAJ | 학술논문

BnaA6. rga-ds是本研究室从甘蓝型油菜突变体ds-1中图位克隆获得的半矮秆基因，它编码一个功能获得型（ gain of function）突变的DELLA蛋白，能够组成型抑制赤霉素信号的转导。蛋白质理化性质的预测显示， BnaA6. rga-ds及其野生型蛋白BnaA6. RGA均为酸性蛋白。将BnaA6. RGA与不同物种同源蛋白的氨基酸序列比对发现，其C端的保守性高于N段。蛋白质结构的预测显示α-螺旋和无规卷曲是其二级和三级结构的主要元件。系统进化分析表明，BnaA6. RGA与十字花科植物拟南芥、小盐芥和荠菜等的同源蛋白具有很高的相似性。BLAST分析表明甘蓝型油菜基因组中共包括4个拷贝的BnaRGA基因。亚细胞定位研究结果显示，BnaA6. RGA及BnaA6. rga-ds均定位于细胞核，它们可能参与下游基因的转录调控。通过农杆菌介导的方法将BnaA6. rga-ds转化甘蓝型油菜品种Westar，获得13株阳性转基因植株，出现明显矮化表型，表明BnaA6. rga-ds基因具有调控油菜株高的能力。
BnaA6. rga-ds시본연구실종감람형유채돌변체ds-1중도위극륭획득적반왜간기인，타편마일개공능획득형（ gain of function）돌변적DELLA단백，능구조성형억제적매소신호적전도。단백질이화성질적예측현시， BnaA6. rga-ds급기야생형단백BnaA6. RGA균위산성단백。장BnaA6. RGA여불동물충동원단백적안기산서렬비대발현，기C단적보수성고우N단。단백질결구적예측현시α-라선화무규권곡시기이급화삼급결구적주요원건。계통진화분석표명，BnaA6. RGA여십자화과식물의남개、소염개화제채등적동원단백구유흔고적상사성。BLAST분석표명감람형유채기인조중공포괄4개고패적BnaRGA기인。아세포정위연구결과현시，BnaA6. RGA급BnaA6. rga-ds균정위우세포핵，타문가능삼여하유기인적전록조공。통과농간균개도적방법장BnaA6. rga-ds전화감람형유채품충Westar，획득13주양성전기인식주，출현명현왜화표형，표명BnaA6. rga-ds기인구유조공유채주고적능력。
A semi-dwarf gene BnaA6 . rga -ds was previously obtained from Brassica napus mutant ds -1 through map-based cloning. It encodes a gain-of-function mutant DELLA protein that constitutively represses the signal transduction of gibberellin acid(GA). In this research,it was predicted that both BnaA6. rga-ds and its wild-type BnaA6. RGA were acidic proteins. Sequence alignment of amino acid showed that BnaA6. RGA and its homologues of other plant species had more conservative C-termini than their N termini. Prediction of seconda-ry and tertiary structures indicated that the protein mainly contained α-helixes and random coils. Phylogenetic a-nalysis revealed that BnaA6. RGA had high homology to RGAs from other Brassicaceae plants,including Arabidop-sis,Thellungiella halophila and Capsella rubella. BLAST in B. napus genome showed that BnaRGAs had 4 copies. Subcellular localization showed that both BnaA6. RGA and BnaA6. rga-ds were located in nucleus. It suggested their function as transcription regulators of downstream genes. BnaA6. rga-ds gene was transformed into B. napus cv Westar by Agrobacterium-mediated method,13 positive transgenic plants were obtained and dwarf phenotype were found. These results suggested that BnaA6. rga-ds gene could regulate the height of Brassicaceae plants.