CAJ | 학술논문

为满足掺伪识别要求，采用固相萃取分离富集食用油中大豆苷元，优化液相色谱质谱条件，建立食用油中大豆苷元液相色谱串联质谱的检测方法。该方法对大豆苷元检测的线性方程为y=8544.77x+58269.4，相关系数为0.9985，回收率为97.6%~117.2%，相对标准偏差小于8.3%，方法检出限为0.02μg/kg，定量限为0.06μg/kg。利用建立的大豆苷元检测方法检测植物油大豆苷元含量，结果表明仅大豆油含有大豆苷元，在花生油、菜籽油和芝麻油中未检出大豆苷元，该检测技术能够对植物油大豆苷元成分定量检测并可进行保真掺伪鉴别，为保证植物油质量、安全消费提供关键技术支撑。
위만족참위식별요구，채용고상췌취분리부집식용유중대두감원，우화액상색보질보조건，건립식용유중대두감원액상색보천련질보적검측방법。해방법대대두감원검측적선성방정위y=8544.77x+58269.4，상관계수위0.9985，회수솔위97.6%~117.2%，상대표준편차소우8.3%，방법검출한위0.02μg/kg，정량한위0.06μg/kg。이용건립적대두감원검측방법검측식물유대두감원함량，결과표명부대두유함유대두감원，재화생유、채자유화지마유중미검출대두감원，해검측기술능구대식물유대두감원성분정량검측병가진행보진참위감별，위보증식물유질량、안전소비제공관건기술지탱。
A method was developed for determination of daidzein in vegetable oil by solid phase extraction-high performance liquid chromatography( HPLC)tandem mass spectrometry. The extraction procedure and liquid chromatography tandem mass spectrometry conditions were optimized. The linear regress equation was y=8 544. 77x+58 269. 4,with correlation coefficient of 0. 998 5. The limit of detection and limit of quantification were 0. 02μg/kg and 0. 06μg/kg,respectively. The recoveries of daidzein in oil samples were in the range of 97. 6 -117. 2%with RSD less than 8. 3%. The results showed that only soybean oil contained daidzein. The proposed method could be used in detecting daidzein and adulteration in vegetable oil,which further provide critical technical sup-ports to ensure the vegetable oil quality and consumer security.