CAJ | 학술논문

为建立大豆脂肪酸组分的绝对定量方法，采用加热甲酯化提取法和气相色谱分析法（ GC），以5种脂肪酸甲酯（棕榈酸甲酯、硬脂酸甲酯、油酸甲酯、亚油酸甲酯和亚麻酸甲酯）为标准样品，在制定5种脂肪酸甲酯组分的标准曲线（ R2﹥0.99）和回归方程的基础上，建立了大豆脂肪酸组分的定量测定方法。该方法可以准确检测大豆籽粒中脂肪酸组分的绝对含量。通过对4个油份含量不同的大豆品种脂肪酸含量测定以及与粗脂肪含量的比较分析发现，该方法可显著提高籽粒中的脂肪酸提取率和检测效率，其检测的总脂肪酸含量占总油脂含量的94%以上。该方法不仅能检测样品中5种脂肪酸组分的相对百分比含量，还可以准确计算出籽粒中各个脂肪酸组分的绝对含量，对大豆脂肪酸检测及育种具有重要意义。
위건립대두지방산조분적절대정량방법，채용가열갑지화제취법화기상색보분석법（ GC），이5충지방산갑지（종려산갑지、경지산갑지、유산갑지、아유산갑지화아마산갑지）위표준양품，재제정5충지방산갑지조분적표준곡선（ R2﹥0.99）화회귀방정적기출상，건립료대두지방산조분적정량측정방법。해방법가이준학검측대두자립중지방산조분적절대함량。통과대4개유빈함량불동적대두품충지방산함량측정이급여조지방함량적비교분석발현，해방법가현저제고자립중적지방산제취솔화검측효솔，기검측적총지방산함량점총유지함량적94%이상。해방법불부능검측양품중5충지방산조분적상대백분비함량，환가이준학계산출자립중각개지방산조분적절대함량，대대두지방산검측급육충구유중요의의。
To develop an absolute quantitative method for fatty acid determination,a quantitative method for fatty acid determination was established through heated -methylation extraction method and gas chromatography ( GC)analysis. Five individual fatty acid methyl esters( i. e. methyl palmitate,methyl stearate,methyl oleate, methyl linoleate and methyl linolenate)were used as the standard samples. According to the standard curves( R2 ﹥0. 99)and the regression equations of five kinds of fatty acid methyl esters,the absolute concentration of fatty acid of soybean seeds could be determined accurately. Through determination of fatty acids in four soybean varieties with different oil content and comparison of the total fatty acid contents with the total fat contents of them,we suggested the extraction efficiency and detection reproducibility of fatty acid could be significantly improved. In addition,the total concentration of five fatty acid detected by this method accounted for more than 94% of total fat content in soy-bean seeds. It concluded that this method could be used to detect not only the relative percentage content but also the absolute concentration of individual fatty acids in soybean seeds,which could play an important role in soybean fatty acid determination and breeding.