CAJ | 학술논문

目的研究脂联素受体1基因rs1139646C/G和rs10920531C/A单核苷酸多态性与中国汉族多囊卵巢综合征(PCOS)妇女的相关性.方法 PCOS组和对照组各80例,PCR分别扩增rs1 139646C/G和rs 10920531 C/A上下游各250个碱基,PCR产物直接基因测序.结果 PCOS组和对照组的rs1139646C/G位点中,全部为基因型CC的纯合子,两组的基因型频率、等位基因频率差异无统计学意义(P＞0.05).PCOS组和对照组的rs10920531C/A位点基因型频率、等位基因频率差异无统计学意义(P =0.683、0.580),两个位点的遗传连锁不平衡分析差异也无统计学意义(x2=0.307,Fisher'sP=0.579610,Pearson'sP=0.579608).结论脂联素受体1基因rs1139646C/G和rs10920531C/A单核苷酸多态性与中国汉族PCOS妇女的发病无关,遗传连锁不平衡分析与PCOS无关,中国汉族人群中rs1139646C/G是比较稳定的位点,未发现该位点的突变,但最终结论有待进一步研究论证.
목적 연구지련소수체1기인rs1139646C/G화rs10920531C/A단핵감산다태성여중국한족다낭란소종합정(PCOS)부녀적상관성.방법 PCOS조화대조조각80례,PCR분별확증rs1 139646C/G화rs 10920531 C/A상하유각250개감기,PCR산물직접기인측서.결과 PCOS조화대조조적rs1139646C/G위점중,전부위기인형CC적순합자,량조적기인형빈솔、등위기인빈솔차이무통계학의의(P＞0.05).PCOS조화대조조적rs10920531C/A위점기인형빈솔、등위기인빈솔차이무통계학의의(P =0.683、0.580),량개위점적유전련쇄불평형분석차이야무통계학의의(x2=0.307,Fisher'sP=0.579610,Pearson'sP=0.579608).결론 지련소수체1기인rs1139646C/G화rs10920531C/A단핵감산다태성여중국한족PCOS부녀적발병무관,유전련쇄불평형분석여PCOS무관,중국한족인군중rs1139646C/G시비교은정적위점,미발현해위점적돌변,단최종결론유대진일보연구론증.
Objective To study the correlation of the adiponectin receptor 1 gene rs1139646C/G and rs10920531C/A single nucleotide polymorphisms with Chinese Han women with polycystic ovary syndrome (PCOS).Methods For PCOS group (n =80) and control group (n =80),polymerase chain reaction (PCR) was used to amplify the upstream and downstream 250 bases of rsl139646C/G and rs10920531C/A,respectively.PCR products were directly sequenced.Results For rs1139646C/G loci sites between PCOS and control groups,all were the homozygous genotype CC,and no statistical significance was found for genotype frequency and allele frequency between two groups.For rs10920531C/A loci sites between PCOS and control groups,no statistical significance was found for genotype frequency and allele frequency between two groups (P =0.683,0.580),and no statistical significance was found for two loci with genetic linkage disequilibrium analysis (x2 =0.307,Fisher 's P =0.579610,Pearson' s P =0.579608).Conclusions Adiponectin receptor 1 gene rs1139646C/G and rs10920531C/A single nucleotide polymorphisms have nothing to do with Chinese Han PCOS women.Genetic linkage disequilibrium analysis has nothing to do with PCOS.The rs1139646C/G in Chinese Han population is relatively stable site,and no locus mutation is found.