CAJ | 학술논문

为阐明甘蓝型油菜BnSGS3抗病毒功能，用5＇-RACE和巢氏PCR方法从油菜中克隆了BnSGS3。该基因全长2638bp，包含4个内含子和1个1824bp的完整开放阅读框。序列对比发现，BnSGS3与其他SGS3基因同源性在58.9%~97.5%之间。构建BnSGS3过表达载体（ BnSGS3-Ov）和干扰表达载体（ BnSGS3-Si），通过浸花（ Floral-dip）法转化拟南芥，经除草剂筛选及PCR检测，获得2种转基因拟南芥各30株。拟南芥接种黄瓜花叶病毒（ CMV）的抗性鉴定显示，转BnSGS3-Ov植株发病轻，株型基本正常，后期能正常开花结籽，对CMV表现中抗；而转BnSGS3-Si植株发病重，不能正常开花结籽。接种油菜花叶病毒（ YoMV）后，2种转基因和非转基因拟南芥均对YoMV表现感病，说明BnSGS3对CMV有一定抗性，但对YoMV无明显抗性。
위천명감람형유채BnSGS3항병독공능，용5＇-RACE화소씨PCR방법종유채중극륭료BnSGS3。해기인전장2638bp，포함4개내함자화1개1824bp적완정개방열독광。서렬대비발현，BnSGS3여기타SGS3기인동원성재58.9%~97.5%지간。구건BnSGS3과표체재체（ BnSGS3-Ov）화간우표체재체（ BnSGS3-Si），통과침화（ Floral-dip）법전화의남개，경제초제사선급PCR검측，획득2충전기인의남개각30주。의남개접충황과화협병독（ CMV）적항성감정현시，전BnSGS3-Ov식주발병경，주형기본정상，후기능정상개화결자，대CMV표현중항；이전BnSGS3-Si식주발병중，불능정상개화결자。접충유채화협병독（ YoMV）후，2충전기인화비전기인의남개균대YoMV표현감병，설명BnSGS3대CMV유일정항성，단대YoMV무명현항성。
To understand the anti-virus function of SGS3 gene in Brassica napus,gene BnSGS3 was cloned from rapeseed cultivar Zhongshuang 6 by 5'-RACE and nested PCR. Sequence analysis showed that BnSGS3 in-cludes 2 638bp,4 introns and a 1 824bp complete ORF. DNAMAN comparison result showed that the similarities between BnSGS3 and other SGS3 genes were 58 . 9% to 97 . 5%. Over-expressing vector BnSGS3-Ov and inter-fering vector BnSGS3-Si were constructed and transformed into Arabidopsis thaliana Columbia ecotype by floral-dip method. 30 transgenic plants of each vector were obtained verified by herbicide spray and PCR identification. After cucumber mosaic virus( CMV)inoculation,BnSGS3-Ov transgenic Arabidopsis plants showed moderate re-sistance to CMV with slighter symptoms than those from BnSGS3-Si transgenic plants. These BnSGS3-Ov plants could grow and flower normally. But BnSGS3-Si plants were seriously infected by CMV with abnormal plants and no flower. Youcai mosaic virus( YoMV)inoculation result showed that both transgenic and wild type plants were susceptive. It suggested that BnSGS3 gene conferred moderate resistance to CMV,but no obvious resistance to Yo-MV.