CAJ | 학술논문

D-塔格糖是一种天然低热量甜味剂。 L-阿拉伯糖异构酶（L-AI）是生物法异构化D-半乳糖为D-塔格糖的关键酶。本研究从腌菜和泡菜中分离出一批乳酸菌，经薄层色谱法初筛和改良半胱氨酸咔唑法复筛，获得一株高产D-塔格糖的乳酸菌，其发酵粗酶液中L-阿拉伯糖异构酶酶活达13.95 U/mL，经16S rDNA序列比对及生化特征分析，该菌被鉴定并命名为Lactobacillus plantarum WU14。研究结果可为生物转化D-塔格糖达到工业化生产奠定基础。
D-탑격당시일충천연저열량첨미제。 L-아랍백당이구매（L-AI）시생물법이구화D-반유당위D-탑격당적관건매。본연구종업채화포채중분리출일비유산균，경박층색보법초사화개량반광안산잡서법복사，획득일주고산D-탑격당적유산균，기발효조매액중L-아랍백당이구매매활체13.95 U/mL，경16S rDNA서렬비대급생화특정분석，해균피감정병명명위Lactobacillus plantarum WU14。연구결과가위생물전화D-탑격당체도공업화생산전정기출。
D-tagatose is a kind of natural sweetener with low-calorie.L-arabinose isomerase is the key enzyme for D-galactose isomerization to D-tagatose by biological method.In this study lactic acid bacteria strains were isolated from pickled vegetables and pickled cabbage.A lactic acid bacteria strain with high yield of D-tagatose was screened by the thin layer chromatography and modified cysteine carbazole method from the lactic acid bacteria strains and its L-arabinose isomerase activity was 13.95 U/mL after analysed.This strain was named Lactobacillus plantarum WU14 based on the se-quence analysis of 16S rDNA and biochemical characteristics.The result of this study could lay the foundation for the bio-conversion D-tagatose industrial production.