CAJ | 학술논문

目的：观察苦参碱对哮喘大鼠 Th1／Th2失衡以及嗜酸细胞中细胞因子转导抑制因子3（SOCS3）表达的影响，探讨苦参碱治疗哮喘气道炎症的作用机制。方法以健康大鼠作为对照组，卵清蛋白致敏法构建大鼠哮喘模型后随机分为 A 组、B 组、C 组。A 组大鼠不接受任何治疗；B、C 组大鼠分别接受低剂量和高剂量的苦参碱治疗。分析各组大鼠肺组织中的嗜酸性粒细胞（EOS）计数、杯状细胞百分比、炎性细胞浸润积分。对比各组大鼠支气管肺泡灌洗液（BAFL）中IL-4、干扰素（INF）-γ水平，计算 INF-γ／IL4比值。荧光实时定量 PCR 技术（qRT-PCR）和蛋白质免疫印迹（Western blot）技术分析各组大鼠肺组织中 SOCS3 mRNA 与蛋白水平的表达变化。结果A 组大鼠出现明显的炎性反应，BAFL 中的 INF-γ水平低于对照组，IL-4水平高于对照组，差异均具有统计学意义。各组 INF-γ／IL-4比值分别为3．90±0．30、0．61±0．08、1．71±0．54、2．15±0．21，各组比较差异有统计学意义（P ＜0．05）。qRT-PCR 检测发现，与对照组比较，A 组大鼠肺组织 SOCS3的 mRNA 表达水平显著增高（P ＜0．05），而 B 组、C 组大鼠肺组织 SOCS3的 mRNA 表达水平明显低于 A 组（P ＜0．05）。SOCS3蛋白水平的变化与 mRNA 水平的变化一致。结论苦参碱可以抑制支气管哮喘大鼠炎症反应、调节 Th1／Th2免疫平衡，该作用的机制与 SOCS3的表达水平有关。
목적：관찰고삼감대효천대서 Th1／Th2실형이급기산세포중세포인자전도억제인자3（SOCS3）표체적영향，탐토고삼감치료효천기도염증적작용궤제。방법이건강대서작위대조조，란청단백치민법구건대서효천모형후수궤분위 A 조、B 조、C 조。A 조대서불접수임하치료；B、C 조대서분별접수저제량화고제량적고삼감치료。분석각조대서폐조직중적기산성립세포（EOS）계수、배상세포백분비、염성세포침윤적분。대비각조대서지기관폐포관세액（BAFL）중IL-4、간우소（INF）-γ수평，계산 INF-γ／IL4비치。형광실시정량 PCR 기술（qRT-PCR）화단백질면역인적（Western blot）기술분석각조대서폐조직중 SOCS3 mRNA 여단백수평적표체변화。결과A 조대서출현명현적염성반응，BAFL 중적 INF-γ수평저우대조조，IL-4수평고우대조조，차이균구유통계학의의。각조 INF-γ／IL-4비치분별위3．90±0．30、0．61±0．08、1．71±0．54、2．15±0．21，각조비교차이유통계학의의（P ＜0．05）。qRT-PCR 검측발현，여대조조비교，A 조대서폐조직 SOCS3적 mRNA 표체수평현저증고（P ＜0．05），이 B 조、C 조대서폐조직 SOCS3적 mRNA 표체수평명현저우 A 조（P ＜0．05）。SOCS3단백수평적변화여 mRNA 수평적변화일치。결론고삼감가이억제지기관효천대서염증반응、조절 Th1／Th2면역평형，해작용적궤제여 SOCS3적표체수평유관。
Objective To observe the effects of matrine on Th1 /Th2 balance and suppressor of cytokine signaling 3 (SOCS3)expression in eosinophil of asthmatic mats and to explore mechanisms of matrine in treating infectious airway of asthmatics.Methods Rats,ovalbumin-sensitized to establish asthma model,were randomly divided into three groups,vehicle(group A),low-dose matrine treatment (group B)and high-dose matrine treatment (group C).Normal rats were kept as control group.The eosinophil count,goblet cells percentage,inflammatory cell infiltration in rat lung were analyzed and scored by morphological examination.IL-4 and INF-γlevel in BALF were determined by ELISA and INF-γ/IL-4 ratio was further calculated.Furthermore,the expression of SOCS3 in mRNA and protein level was detected by qRT-PCR and Western blotting,respectively.Results Compare to control group,inflammation was evident in group A rats.Eosinophil count significantly elevated to 14.6 ±1.9 per high power field,with gob-let cells increased to (67.6 ±5.1)%,and inflammatory cell infiltration scored 3.7 ±0.7(all P <0.05). Eosinophil count,goblet cell percentage and inflammatory cell infiltration score were significantly lower in group B and C,compare to group A(P <0.05).Compared to control group,group A exhibited a lower INF-γlevel and a higher IL-4 level(P <0.05).INF-γlevel in group B and C were higher while IL-4 level were lower,compare to group A.The INF-γ/IL-4 ratio were 3.90 ±0.30,0.61 ±0.08,1.71 ±0.54,and 2.15 ±0.21 in group A,B and C,respectively.Meanwhile,SOCS3 mRNA level in rat lung tissue was elevated in group A,compare to control group.Matrine treatment decreased SOCS3 expression in group B and C.Similar trend was found in SOCS3 protein level.Conclusion Matrine may exhibit its anti-inflam-matory effect by inhibiting SOCS3 expression and maintaining Th1 /Th2 balance in asthmatic rats.