CAJ | 학술논문

目的：研究不同浓度的睾酮对体外平滑肌源性泡沫细胞形成的影响，及其促进胆固醇外转运蛋白三磷酸腺苷结合盒转运体 A1（ABCA1）的表达机制。方法采用不同浓度（20、40、80μg／ml）的睾酮体外干预平滑肌源性泡沫细胞，油红 O 染色观察泡沫细胞的形成，实时荧光定量聚合酶链反应（Real-time PCR）和 Western blot 检测肝细胞 X 受体α（LXRα）、过氧化物酶体增殖物激活受体γ（PPARγ）和 ABCA1基因在转录和翻译水平的表达。结果不同浓度睾酮能明显抑制泡沫细胞内脂质的蓄积，在转录水平和翻译水平能够促进 PPARγ、LXRα和 ABCA1基因的表达。结论睾酮在体外可以抑制泡沫细胞的形成，此作用可能通过 PPAR γ／LXR α／ABCA1途径实现。
목적：연구불동농도적고동대체외평활기원성포말세포형성적영향，급기촉진담고순외전운단백삼린산선감결합합전운체 A1（ABCA1）적표체궤제。방법채용불동농도（20、40、80μg／ml）적고동체외간예평활기원성포말세포，유홍 O 염색관찰포말세포적형성，실시형광정량취합매련반응（Real-time PCR）화 Western blot 검측간세포 X 수체α（LXRα）、과양화물매체증식물격활수체γ（PPARγ）화 ABCA1기인재전록화번역수평적표체。결과불동농도고동능명현억제포말세포내지질적축적，재전록수평화번역수평능구촉진 PPARγ、LXRα화 ABCA1기인적표체。결론고동재체외가이억제포말세포적형성，차작용가능통과 PPAR γ／LXR α／ABCA1도경실현。
Objective To explore the mechanism of testosterone on the formation of foam cells in vitro and the expression of cholesterol transport protein ATP-binding cassette sub-familyA member1 (ABCA1).Methods The rats vascular smooth muscle cell-derived foam cells were treated with different concentrations of testosterone(20 μg/ml,40 μg/ml and 80 μg/ml)in vitro and then stained by oil red O. The expression of LXRα,PPARγand ABCA1 were detected by Real-time polymerase chain reaction (PCR)and Western blotting.Results Testosterone(80 μg/ml)promoted cholesterol transport protein ABCA1 expression through increased the expression of peroxisome proliferator-activated receptor-γ(PPARγ)and the liver X receptor-α(LXRα).Conclusion Testosterone could inhibite the formation of rats vascular smooth muscle-derived foam cell by PPARγ/LXRα/ABCA1 pathway.