CAJ | 학술논문

目的探讨多发性骨髓瘤(MM)患者血清铁调素及白细胞介素(IL)-6表达水平及与贫血的相关性关系.方法选取江苏省太仓市第一人民医院血液肿瘤科于2010年1月至2014年12月收治的32例MM患者为研究对象,并纳入研究组.选取同期于本院体检的健康个体20例纳入对照组.本研究遵循的程序符合江苏省太仓市第一人民医院人体试验委员会所制定的伦理学标准,得到该委员会批准,征得受试对象的知情同意,并与之签署临床研究知情同意书.应用酶联免疫吸附试验(ELISA)方法检测两组受试者血清铁调素与IL-6水平,应用XE2100全自动血液分析仪检测两组受试者血红蛋白(Hb)水平,应用化学发光法检测两组受试者铁蛋白水平,并分析MM患者血清铁调素水平与IL-6、铁蛋白及Hb水平相关性关系.结果 ①多发性骨髓瘤患者的铁调素、IL-6及铁蛋白的水平分别为(6.06±4.78)μg/L、(35.23±31.37) ng/L和(351.52±256.93) μg/L,均显著高于对照组,且差异均有统计学意义(P＜0.05).②铁调素水平与IL-6、铁蛋白浓度水平呈正相关关系(r=0.545,0.593;P=0.007,0.003),铁调素水平则与Hb水平呈负相关关系(r=-0.494,P=0.017).结论 MM患者血清铁调素及IL-6表达水平均增高,铁调素及IL-6可能参与患者慢性病贫血的发生.
목적 탐토다발성골수류(MM)환자혈청철조소급백세포개소(IL)-6표체수평급여빈혈적상관성관계.방법 선취강소성태창시제일인민의원혈액종류과우2010년1월지2014년12월수치적32례MM환자위연구대상,병납입연구조.선취동기우본원체검적건강개체20례납입대조조.본연구준순적정서부합강소성태창시제일인민의원인체시험위원회소제정적윤리학표준,득도해위원회비준,정득수시대상적지정동의,병여지첨서림상연구지정동의서.응용매련면역흡부시험(ELISA)방법검측량조수시자혈청철조소여IL-6수평,응용XE2100전자동혈액분석의검측량조수시자혈홍단백(Hb)수평,응용화학발광법검측량조수시자철단백수평,병분석MM환자혈청철조소수평여IL-6、철단백급Hb수평상관성관계.결과 ①다발성골수류환자적철조소、IL-6급철단백적수평분별위(6.06±4.78)μg/L、(35.23±31.37) ng/L화(351.52±256.93) μg/L,균현저고우대조조,차차이균유통계학의의(P＜0.05).②철조소수평여IL-6、철단백농도수평정정상관관계(r=0.545,0.593;P=0.007,0.003),철조소수평칙여Hb수평정부상관관계(r=-0.494,P=0.017).결론 MM환자혈청철조소급IL-6표체수평균증고,철조소급IL-6가능삼여환자만성병빈혈적발생.
Objective To investigate the expression levels of hepcidin,interleukin (IL)-6 and their relation with anemia in multiple myeloma (MM) patients.Methods From January 2010 to December 2014,a total of 32 MM patients who were diagnosed as MM in the First People's Hospital of Taicang were collected into this study,as study group (n=32).And 20 cases of healthy person in the same period were collected into control group.The study protocol was approved by the Ethical Review Board of Investigation in Human at the First People's Hospital of Taicang.Informed consent was obtained from all participants.The serum levels of hepcidin and IL-6 were tested by enzyme-linked immuosorbent assay (ELISA).The levels of hemoglobin (Hb) were detected by XE2100 fully automatic hematology analyzer.The serum ferritin were measured by chemiluminescence method in two groups.The relation between hepcidin,IL-6 and Hb was analyzed.Results ①The serum levels of hepcidin,IL-6 and serum ferritin in MM patients were (6.06±4.78) μg/L,(35.23±31.37) ng/L and (351.52±256.93)μg/L,which were significantly higher than those of control group (P＜0.05).②The expression level of hepcidin was positively correlated with serum ferritin and IL-6 levels (r=0.545,0.593;P=0.007,0.003),and it was negatively correlated with Hb level (r=-0.494,P=0.017).Conclusions The expression levels of hepcidin and IL-6 in MM patients were increased and may play an etiologic role in anemia of chronic disease.