国际输血及血液学杂志
國際輸血及血液學雜誌
국제수혈급혈액학잡지
INTERNATIONAL JOURNAL OF BLOOD TRANSFUSION AND HEMATOLOGY
2015年
4期
308-311
,共4页
血小板%低温保存%血液保存
血小闆%低溫保存%血液保存
혈소판%저온보존%혈액보존
Blood platelets%Cryopreservation%Blood preservation
目的 探讨血小板采集后不同时间冷冻保存对其体外功能的影响.方法 2011年12月至2013年12月东营市血液中心采集的180袋血小板作为研究对象.采用计算机随机法将其分为两组:12 h内冷冻保存组(n=90):血小板于采集12 h内冷冻保存;72 h后的冷冻保存组(n=90):血小板于采集后常规保存72 h后再冷冻保存.两组血小板采集及复融的方法均相同,且冷冻前血小板计数等资料差异无统计学意义(P＞0.05).两组血小板冷冻保存时间均为1年,1年后取样10 mL用于其体外功能检测.血小板体外功能检测项目包括:CD62P表达、黏附功能、低渗休克反应(HSR)值、聚集功能.分别对两组血小板上述指标进行比较.结果 12 h内冷冻保存组与72 h后的冷冻保存组血小板冷冻保存1年后,血小板计数、CD62P表达率、CD62P再表达率、黏附率、HRS分别为(2.5±0.4)×109/L比(2.5±0.4)×109/L、(20.2±4.6)％比(19.9±4.3)％、(71.3±4.7)％比(70.8±4.5)％、(43.5±6.7)％比(52.5±5.5)％、(53.4±11.1)％比(52.8±10.5)％,两组血小板上述各项功能指标比较,差异均无统计学意义(u=0.00,0.05,0.08,0.04,1.04;P＞0.05).两组血小板分别加入胶原蛋白、瑞斯托霉素、凝血酶及花生四烯酸4种血小板聚集诱导剂后,最大聚集率分别为(35.6±12.1)％比(33.4±14.0)％、(23.5±11.2)％比(21.5±12.1)％、(47.0±8.8)％比(46.8±7.9)％、(40.2±26.3)％比(38.4±23.3)％.两组血小板加入各种聚集诱导剂后,最大聚集率比较,差异亦无统计学意义(u=0.12,0.12,0.02,0.05;P＞0.05).结论 液态血小板常规保存72 h后,再进行-80℃深低温冷冻保存的方法,即能满足临床对新鲜液态血小板的需要,又可保障临床应急用血的需求.
目的 探討血小闆採集後不同時間冷凍保存對其體外功能的影響.方法 2011年12月至2013年12月東營市血液中心採集的180袋血小闆作為研究對象.採用計算機隨機法將其分為兩組:12 h內冷凍保存組(n=90):血小闆于採集12 h內冷凍保存;72 h後的冷凍保存組(n=90):血小闆于採集後常規保存72 h後再冷凍保存.兩組血小闆採集及複融的方法均相同,且冷凍前血小闆計數等資料差異無統計學意義(P＞0.05).兩組血小闆冷凍保存時間均為1年,1年後取樣10 mL用于其體外功能檢測.血小闆體外功能檢測項目包括:CD62P錶達、黏附功能、低滲休剋反應(HSR)值、聚集功能.分彆對兩組血小闆上述指標進行比較.結果 12 h內冷凍保存組與72 h後的冷凍保存組血小闆冷凍保存1年後,血小闆計數、CD62P錶達率、CD62P再錶達率、黏附率、HRS分彆為(2.5±0.4)×109/L比(2.5±0.4)×109/L、(20.2±4.6)％比(19.9±4.3)％、(71.3±4.7)％比(70.8±4.5)％、(43.5±6.7)％比(52.5±5.5)％、(53.4±11.1)％比(52.8±10.5)％,兩組血小闆上述各項功能指標比較,差異均無統計學意義(u=0.00,0.05,0.08,0.04,1.04;P＞0.05).兩組血小闆分彆加入膠原蛋白、瑞斯託黴素、凝血酶及花生四烯痠4種血小闆聚集誘導劑後,最大聚集率分彆為(35.6±12.1)％比(33.4±14.0)％、(23.5±11.2)％比(21.5±12.1)％、(47.0±8.8)％比(46.8±7.9)％、(40.2±26.3)％比(38.4±23.3)％.兩組血小闆加入各種聚集誘導劑後,最大聚集率比較,差異亦無統計學意義(u=0.12,0.12,0.02,0.05;P＞0.05).結論 液態血小闆常規保存72 h後,再進行-80℃深低溫冷凍保存的方法,即能滿足臨床對新鮮液態血小闆的需要,又可保障臨床應急用血的需求.
목적 탐토혈소판채집후불동시간냉동보존대기체외공능적영향.방법 2011년12월지2013년12월동영시혈액중심채집적180대혈소판작위연구대상.채용계산궤수궤법장기분위량조:12 h내냉동보존조(n=90):혈소판우채집12 h내냉동보존;72 h후적냉동보존조(n=90):혈소판우채집후상규보존72 h후재냉동보존.량조혈소판채집급복융적방법균상동,차냉동전혈소판계수등자료차이무통계학의의(P＞0.05).량조혈소판냉동보존시간균위1년,1년후취양10 mL용우기체외공능검측.혈소판체외공능검측항목포괄:CD62P표체、점부공능、저삼휴극반응(HSR)치、취집공능.분별대량조혈소판상술지표진행비교.결과 12 h내냉동보존조여72 h후적냉동보존조혈소판냉동보존1년후,혈소판계수、CD62P표체솔、CD62P재표체솔、점부솔、HRS분별위(2.5±0.4)×109/L비(2.5±0.4)×109/L、(20.2±4.6)％비(19.9±4.3)％、(71.3±4.7)％비(70.8±4.5)％、(43.5±6.7)％비(52.5±5.5)％、(53.4±11.1)％비(52.8±10.5)％,량조혈소판상술각항공능지표비교,차이균무통계학의의(u=0.00,0.05,0.08,0.04,1.04;P＞0.05).량조혈소판분별가입효원단백、서사탁매소、응혈매급화생사희산4충혈소판취집유도제후,최대취집솔분별위(35.6±12.1)％비(33.4±14.0)％、(23.5±11.2)％비(21.5±12.1)％、(47.0±8.8)％비(46.8±7.9)％、(40.2±26.3)％비(38.4±23.3)％.량조혈소판가입각충취집유도제후,최대취집솔비교,차이역무통계학의의(u=0.12,0.12,0.02,0.05;P＞0.05).결론 액태혈소판상규보존72 h후,재진행-80℃심저온냉동보존적방법,즉능만족림상대신선액태혈소판적수요,우가보장림상응급용혈적수구.
Objective To explore impact of platelets in vitro function on different time after acquisition for cryopreservation.Methods From December 2011 to December 2013,a total of 180 bags platelets which were collected in Dongying Central Blood Station were included into this study.All the platelets were divided into two groups by computer randomly.Within 12 h frozen group (n=90) included 90 bags platelets,which were frozen stored within 12 h after collection.After 72 h frozen group (n=90) also included 90 bags platelets,which were frozen stored before general preservation for 72 h.Melting and collection methods of platelets in two groups were the same,and there were no statistically significant in platelet counts or other baseline data before cryopreservation in two groups (P＞ 0.05).After one year cryopreservation,platelets were melted and 10 mL platelets were collected for in vitro function tests.Platelets in vitro function tests included CD62P expression rate,adhesion function,hypotonic shock response (HRS) and aggregation function.Above indexes in two groups were compared.Results After one year cryopreservation,the platelet counts,CD62P expression rate,adhesion function and HRS value in two groups were (2.5±0.4) × 109/L vs (2.5±0.4) × 109/L,(20.2±4.6)％ vs (19.9±4.3)％,(71.3± 4.7)％ vs (70.8±4.5)％,(43.5±6.7)％ vs (52.5±5.5)％,and (53.4±11.1)％ vs (52.8±10.5)％,respectively.There were no statistical differences between two groups in each index (u=0.00,0.05,0.08,0.04,1.04;P＞0.05).Max aggregation rate of platelets in two groups after adding with collagen,ristocetin,thrombin and arachidonic acid were (35.6 ± 12.1) ％ vs (33.4 ± 14.0) ％,(23.5 ± 11.2) ％ vs (21.5±12.1)％,(47.0±8.8)％ vs (46.8±7.9)％,and (40.2±26.3)％ vs (38.4± 23.3)％,respectively.There were no statistical differences in max aggregation rate between two groups (u=0.12,0.12,0.02,0.05;P＞0.05).Conclusions The method that platelets frozen stored after 72 h general preservation could not only meet the clinical needs of the fresh liquid platelets,but also protect clinical emergency blood needs.
