CAJ | 학술논문

为获得含有天花粉蛋白（ TCS）的转基因大豆植株，提高大豆的抗病虫能力，进行了植物表达载体的构建及转入大豆的研究。首先构建植物表达载体pC－tPro－TCS－GUS，将其转化E．coli DH5α，经SDS－PAGE结果表明：克隆得到的TCS基因可以在大肠杆菌中表达。通过根癌农杆菌介导法，将TCS基因导入大豆合丰35中，获得了19株T0代转基因大豆，转化率为1．033％。选取T0代种子种植在大田中共获得18株T1代转基因大豆，对获得的T0代和T1代转基因植株进行PCR和PCR－Southern检测，证实外源天花粉蛋白基因己经整合到大豆基因组中。
위획득함유천화분단백（ TCS）적전기인대두식주，제고대두적항병충능력，진행료식물표체재체적구건급전입대두적연구。수선구건식물표체재체pC－tPro－TCS－GUS，장기전화E．coli DH5α，경SDS－PAGE결과표명：극륭득도적TCS기인가이재대장간균중표체。통과근암농간균개도법，장TCS기인도입대두합봉35중，획득료19주T0대전기인대두，전화솔위1．033％。선취T0대충자충식재대전중공획득18주T1대전기인대두，대획득적T0대화T1대전기인식주진행PCR화PCR－Southern검측，증실외원천화분단백기인기경정합도대두기인조중。
In order to obtain transgenic soybean plants,which are resistant to diseases and insects,the ti-chosanthin( TCS) gene was transformed into soybean plants.First the plant expression vector pC-tPro-TCS-GUS was constructed,then transformed into E.coli DH5α.SDS-PAGE results showed that TCS gene could be ex-pressed in E.coli.TCS gene was transformed into the cotyledonary node of soybean Hefeng35 througha grobac-terium-mediated method.The conversion rate was 1.033%with 19 transgenic plants obtained in the T0 genera-tion.Then 18 strains in the T1 generation were harvested after selecting T0 generation seeds which were planted in the field.PCR and PCR-Southern analysis proved that the TCS gene was integrated into the soybean genome both in the T0 andT1 generations.