临床与病理杂志
臨床與病理雜誌
림상여병리잡지
International Journal of Pathology and Clinical Medicine
2015年
z1期
1-6
,共6页
许开武%秦长江%陈志辉%宋新明
許開武%秦長江%陳誌輝%宋新明
허개무%진장강%진지휘%송신명
PARP1%结直肠癌%shRNA%细胞增殖%细胞凋亡
PARP1%結直腸癌%shRNA%細胞增殖%細胞凋亡
PARP1%결직장암%shRNA%세포증식%세포조망
PARP1%colorectal cancer (CRC)%shRNA%proliferation%apoptosis
目的:探究PARP1在结直肠癌的表达情况及其与癌临床病理特征的关系,以及结肠癌细胞株SW620、LoVo细胞PARP1的表达,分析其对结直肠癌细胞株SW620增殖与凋亡的影响。方法:应用免疫组织化学检测39例结直肠癌组织及癌旁正常组织中PARP1的表达;实时荧光定量PCR(QPCR)检测结直肠癌细胞株SW620、LoVo、结肠正常黏膜细胞中PARP1的表达;转染PARP1基因shRNA表达载体后,MTT检测细胞增殖,流式细胞术检测细胞凋亡情况,QPCR、Western blotting分别检测cyclin D1及Caspase-3的基因与蛋白的表达情况。结果:PARP1在结直肠癌组织中的阳性表达率为71.8%,显著高于癌旁正常组织的20.5%(P<0.05)。PARP1在结肠癌细胞株SW620、LoVo的表达显著高于正常结肠黏膜细胞。转染PARP1基因shRNA后,PARP1水平明显减低,SW620细胞增殖能力减弱,凋亡能力增强。结论:PARP1在结直肠癌中高表达,可促进结直肠癌细胞增殖,抑制细胞凋亡。
目的:探究PARP1在結直腸癌的錶達情況及其與癌臨床病理特徵的關繫,以及結腸癌細胞株SW620、LoVo細胞PARP1的錶達,分析其對結直腸癌細胞株SW620增殖與凋亡的影響。方法:應用免疫組織化學檢測39例結直腸癌組織及癌徬正常組織中PARP1的錶達;實時熒光定量PCR(QPCR)檢測結直腸癌細胞株SW620、LoVo、結腸正常黏膜細胞中PARP1的錶達;轉染PARP1基因shRNA錶達載體後,MTT檢測細胞增殖,流式細胞術檢測細胞凋亡情況,QPCR、Western blotting分彆檢測cyclin D1及Caspase-3的基因與蛋白的錶達情況。結果:PARP1在結直腸癌組織中的暘性錶達率為71.8%,顯著高于癌徬正常組織的20.5%(P<0.05)。PARP1在結腸癌細胞株SW620、LoVo的錶達顯著高于正常結腸黏膜細胞。轉染PARP1基因shRNA後,PARP1水平明顯減低,SW620細胞增殖能力減弱,凋亡能力增彊。結論:PARP1在結直腸癌中高錶達,可促進結直腸癌細胞增殖,抑製細胞凋亡。
목적:탐구PARP1재결직장암적표체정황급기여암림상병리특정적관계,이급결장암세포주SW620、LoVo세포PARP1적표체,분석기대결직장암세포주SW620증식여조망적영향。방법:응용면역조직화학검측39례결직장암조직급암방정상조직중PARP1적표체;실시형광정량PCR(QPCR)검측결직장암세포주SW620、LoVo、결장정상점막세포중PARP1적표체;전염PARP1기인shRNA표체재체후,MTT검측세포증식,류식세포술검측세포조망정황,QPCR、Western blotting분별검측cyclin D1급Caspase-3적기인여단백적표체정황。결과:PARP1재결직장암조직중적양성표체솔위71.8%,현저고우암방정상조직적20.5%(P<0.05)。PARP1재결장암세포주SW620、LoVo적표체현저고우정상결장점막세포。전염PARP1기인shRNA후,PARP1수평명현감저,SW620세포증식능력감약,조망능력증강。결론:PARP1재결직장암중고표체,가촉진결직장암세포증식,억제세포조망。
Objective:To investigate PARP1 expression in tumor and adjacent normal tissues from colorectal cancer (CRC) patients, and colon cancer lines SW620 and LoVo, and analyze the relationship between PARP1 expression and clinic pathologic features, and the effect of PARP1 expression on proliferation and apoptosis of SW620 cells. Methods: Immunohistochemistry and QPCR were respectively used to detect PARP1 expression in CRC tissues and adjacent normal intestinal mucosa, and in SW620 and LoVo cells. After transfection with PARP1-shRNA, the proliferation and apoptosis of SW620 cells were detected by MTT and lfow cytometry. hTe gene and protein expressions of cyclinD1 and Caspase-3 were detected by QPCR and Western blotting.Results: The positive expression rate of PARP1 was 71.8% in CRC tissues, signiifcantly higher than 20.5% in adjacent normal intestinal mucosa tissues (P<0.05). hTe expression levels of PARP1 in SW620, LoVo cells were signiifcantly higher than that in normal intestinal mucosa cells. Atfer transfection with PARP1-shRNA, PARP1 expression and cell proliferation were signiifcantly inhibited, while tumor cell apoptosis increased.Conclusion: PARP1 expression is high in CRC, which can promote proliferation and prohibit apoptosis of tumor cells.