中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2015年
8期
547-550
,共4页
郭柏华%杨章林%董国钢%王笑凌%班雨
郭柏華%楊章林%董國鋼%王笑凌%班雨
곽백화%양장림%동국강%왕소릉%반우
结直肠肿瘤%免疫组织化学%逆转录聚合酶链反应%Pokemon%C-myc
結直腸腫瘤%免疫組織化學%逆轉錄聚閤酶鏈反應%Pokemon%C-myc
결직장종류%면역조직화학%역전록취합매련반응%Pokemon%C-myc
Colorectal neoplasms%Immunohistochemistry%Reverse transcriptase polymerase chain reaction%Pokemon%C-myc
目的 检测Pokemon和c-myc在结直肠癌、结直肠腺瘤和正常结直肠黏膜组织中的表达情况,研究结直肠癌组织中Pokemon和c-myc的表达与临床病理因素的关系.方法 收集86例结直肠癌患者肿瘤组织标本,60例结直肠腺瘤组织标本和40例正常结直肠黏膜组织标本.采用免疫组织化学法检测组织中Pokemon和c-myc蛋白表达情况,反转录聚合酶链反应检测组织中Pokemon和c-myc基因表达水平,并分析其与结直肠癌临床病理因素的关系.结果 结直肠癌组织中Pokemon和c-myc蛋白阳性表达率均明显高于结直肠腺瘤组织和正常结直肠黏膜组织[69.8％(60/86)比13.3％(8/60)和5.0％(2/40)、73.3％(63/86)比15.0％(9/60)和2.5％(1/40)],结直肠癌组织中Pokemon和c-myc基因表达水平均明显高于结直肠腺瘤组织和正常结直肠黏膜组织(0.915±0.247比0.358±0.102和0.277±0.085、1.272±0.360比0.398±0.153和0.255 ± 0.097),差异有统计学意义(P＜0.05).结直肠癌组织中Pokemon和c-myc基因的表达水平与肿瘤浸润程度、肿瘤分化程度、淋巴结转移、远处转移和Dukes分期有关,差异有统计学意义(P＜0.05),但与性别、年龄和肿瘤直径无关(P＞0.05).结论 Pokemon和c-myc在结直肠癌组织中高表达,且这种表达与结直肠癌临床病理特征有关,提示Pokemon和c-myc可作为结直肠癌患者临床治疗的评估指标.
目的 檢測Pokemon和c-myc在結直腸癌、結直腸腺瘤和正常結直腸黏膜組織中的錶達情況,研究結直腸癌組織中Pokemon和c-myc的錶達與臨床病理因素的關繫.方法 收集86例結直腸癌患者腫瘤組織標本,60例結直腸腺瘤組織標本和40例正常結直腸黏膜組織標本.採用免疫組織化學法檢測組織中Pokemon和c-myc蛋白錶達情況,反轉錄聚閤酶鏈反應檢測組織中Pokemon和c-myc基因錶達水平,併分析其與結直腸癌臨床病理因素的關繫.結果 結直腸癌組織中Pokemon和c-myc蛋白暘性錶達率均明顯高于結直腸腺瘤組織和正常結直腸黏膜組織[69.8％(60/86)比13.3％(8/60)和5.0％(2/40)、73.3％(63/86)比15.0％(9/60)和2.5％(1/40)],結直腸癌組織中Pokemon和c-myc基因錶達水平均明顯高于結直腸腺瘤組織和正常結直腸黏膜組織(0.915±0.247比0.358±0.102和0.277±0.085、1.272±0.360比0.398±0.153和0.255 ± 0.097),差異有統計學意義(P＜0.05).結直腸癌組織中Pokemon和c-myc基因的錶達水平與腫瘤浸潤程度、腫瘤分化程度、淋巴結轉移、遠處轉移和Dukes分期有關,差異有統計學意義(P＜0.05),但與性彆、年齡和腫瘤直徑無關(P＞0.05).結論 Pokemon和c-myc在結直腸癌組織中高錶達,且這種錶達與結直腸癌臨床病理特徵有關,提示Pokemon和c-myc可作為結直腸癌患者臨床治療的評估指標.
목적 검측Pokemon화c-myc재결직장암、결직장선류화정상결직장점막조직중적표체정황,연구결직장암조직중Pokemon화c-myc적표체여림상병리인소적관계.방법 수집86례결직장암환자종류조직표본,60례결직장선류조직표본화40례정상결직장점막조직표본.채용면역조직화학법검측조직중Pokemon화c-myc단백표체정황,반전록취합매련반응검측조직중Pokemon화c-myc기인표체수평,병분석기여결직장암림상병리인소적관계.결과 결직장암조직중Pokemon화c-myc단백양성표체솔균명현고우결직장선류조직화정상결직장점막조직[69.8％(60/86)비13.3％(8/60)화5.0％(2/40)、73.3％(63/86)비15.0％(9/60)화2.5％(1/40)],결직장암조직중Pokemon화c-myc기인표체수평균명현고우결직장선류조직화정상결직장점막조직(0.915±0.247비0.358±0.102화0.277±0.085、1.272±0.360비0.398±0.153화0.255 ± 0.097),차이유통계학의의(P＜0.05).결직장암조직중Pokemon화c-myc기인적표체수평여종류침윤정도、종류분화정도、림파결전이、원처전이화Dukes분기유관,차이유통계학의의(P＜0.05),단여성별、년령화종류직경무관(P＞0.05).결론 Pokemon화c-myc재결직장암조직중고표체,차저충표체여결직장암림상병리특정유관,제시Pokemon화c-myc가작위결직장암환자림상치료적평고지표.
Objective To investigate the expressions of Pokemon and c-myc in colorectal cancer,colorectal adenomas and normal colorectal mucosa tissues,and demonstrate its relationship with clinicopathological features and prognosis in patients with colorectal cancer.Methods The specimens were taken from colorectal cancer tissue of 86 patients,colorectal adenomas tissue of 60 patients,and 40 normal colorectal mucosa tissue.The expressions of Pokemon and c-myc protein were detected by immunohistochemistry,and the expressions of Pokemon and c-myc gene were detected by reverse transcription polymerase chain reaction (RT-PCR).The correlation between the expressions of Pokemon and c-myc,and clinicopathological features was analyzed.Results The positive expression rates of Pokemon and c-myc protein in colorectal cancer tissue were significantly higher than those in colorectal adenomas tissue and normal colorectal mucosa tissue:69.8％ (60/86) vs.13.3％ (8/60) and 5.0％ (2/40),73.3％ (63/86) vs.15.0％ (9/60) and 2.5％(1/40),and the expression levels of Pokemon and c-myc gene in colorectal cancer tissue were significantly higher than those in colorectal adenomas tissue and normal colorectal mucosa tissue:0.915 ±0.247 vs.0.358 ±0.102 and 0.277 ±0.085,1.272 ±0.360 vs.0.398 ± 0.153 and 0.255 ±0.097,there were statistical differences (P ＜ 0.05).The expression levels of Pokemon and c-myc gene in colorectal cancer tissue had a close correlation with the degree of infiltration,histological differentiation,lymph node metastasis,distant metastasis and Dukes stage,there were statistical differences (P ＜ 0.05),but had no correlation with the sex,age,and tumor diameter (P ＞ 0.05).Conclusions The expression levels of Pokemon and c-myc are higher in colorectal cancer tissue,and the expressions are related to the clinicopathological features of colorectal cancer.The expression levels of Pokemon and c-myc may predict the prognosis in patients with colorectal cancer.
