中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2015年
8期
1183-1186
,共4页
微RNAs/代谢%鼻咽肿瘤/代谢%复发%肿瘤转移%预后
微RNAs/代謝%鼻嚥腫瘤/代謝%複髮%腫瘤轉移%預後
미RNAs/대사%비인종류/대사%복발%종류전이%예후
MicroRNAs/ME%Nasopharyngeal neoplasms/ME%Recurrence%Neoplasm metastasis%Prognosis
目的 探讨microRNA-21、microRNA-143和microRNA-145在鼻咽癌患者血清中的表达及其在复发转移和预后评估中的价值.方法 选取2012年1月至2014年1月在本院肿瘤内科和头颈外科住院的鼻咽癌患者80例,同期80例健康志愿者作为对照.采用实时荧光定量PCR(RT-PCR)技术检测其血清中microRNA-21、microRNA-143和microRNA-145的表达水平.结果 鼻咽癌患者microRNA-21表达量显著高于健康对照组;鼻咽癌患者microRNA-143和microRNA-145表达量显著低于健康对照组,差异均有统计学意义(P＜0.05),同时与组织分化及侵袭转移的程度有关.结论 鼻咽癌患者血清中microRNA-21水平表达上升,microRNA-143和microRNA-145水平表达下降,它们在鼻咽癌组织分化及侵袭转移的发生、发展过程中发挥着重要作用,microRNAs可以作为鼻咽癌的辅助诊断、复发转移和预后评估的一种新指标.
目的 探討microRNA-21、microRNA-143和microRNA-145在鼻嚥癌患者血清中的錶達及其在複髮轉移和預後評估中的價值.方法 選取2012年1月至2014年1月在本院腫瘤內科和頭頸外科住院的鼻嚥癌患者80例,同期80例健康誌願者作為對照.採用實時熒光定量PCR(RT-PCR)技術檢測其血清中microRNA-21、microRNA-143和microRNA-145的錶達水平.結果 鼻嚥癌患者microRNA-21錶達量顯著高于健康對照組;鼻嚥癌患者microRNA-143和microRNA-145錶達量顯著低于健康對照組,差異均有統計學意義(P＜0.05),同時與組織分化及侵襲轉移的程度有關.結論 鼻嚥癌患者血清中microRNA-21水平錶達上升,microRNA-143和microRNA-145水平錶達下降,它們在鼻嚥癌組織分化及侵襲轉移的髮生、髮展過程中髮揮著重要作用,microRNAs可以作為鼻嚥癌的輔助診斷、複髮轉移和預後評估的一種新指標.
목적 탐토microRNA-21、microRNA-143화microRNA-145재비인암환자혈청중적표체급기재복발전이화예후평고중적개치.방법 선취2012년1월지2014년1월재본원종류내과화두경외과주원적비인암환자80례,동기80례건강지원자작위대조.채용실시형광정량PCR(RT-PCR)기술검측기혈청중microRNA-21、microRNA-143화microRNA-145적표체수평.결과 비인암환자microRNA-21표체량현저고우건강대조조;비인암환자microRNA-143화microRNA-145표체량현저저우건강대조조,차이균유통계학의의(P＜0.05),동시여조직분화급침습전이적정도유관.결론 비인암환자혈청중microRNA-21수평표체상승,microRNA-143화microRNA-145수평표체하강,타문재비인암조직분화급침습전이적발생、발전과정중발휘착중요작용,microRNAs가이작위비인암적보조진단、복발전이화예후평고적일충신지표.
Objective To investigate the expressions of microRNA-21,microRNA-143,and microRNA-145 in the sera of patients with nasopharyngeal carcinoma (NPC),and their assessment vales in the recurrence,metastasis,and prognosis of NPC patients.Methods From January 2012 to January 2014,80 NPC patients in our hospital Department of Internal Medicine and Head and Neck Surgery was used as tumor group,80 cases of healthy volunteers as control.The real-time fluorescence quantitative polymerase chain reaction (RT-PCR) was used to detect the expression levels of microRNA-21,microRNA-143,and microRNA-145 in the sera.Results Expression of microRNA-21 in NPC patients was significantly higher than that in healthy control group with statistically significant difference (P ＜ 0.05).The expressions of microRNA-143 and microRNA-145 in NPC patients was significantly lower than those in healthy control group with significant difference (P ＜ 0.05).At the same time,those microRNAs were significantly associated with tissue differentiation,invasion,and metastasis.Conclusions Increased microRNA-21 expression level in NPC patients,and decreased expression of microRNA-143 and microRNA-145 in NPC patients play an important role in differentiation,invasion,and metastasis in the development process of NPC.microRNAs can be used as a new index in the auxiliary diagnosis of NPC and the evaluation of recurrence,metastasis,and prognosis evaluation.
