CAJ | 학술논문

目的初步探讨嗜酸粒细胞型的伴有鼻息肉的慢性鼻-鼻窦炎(Eos CRSwNP)发病过程中miRNA对B淋巴细胞刺激因子(BAFF)的调控影响.方法收集2012年1月至2013年2月在本院治疗的Eos CRSwNP患者鼻黏膜标本10例和正常鼻黏膜标本10例.运用生物信息学软件(Pictar、TargetScan及miRanda)预测对BAFF进行调控的miRNA.运用实时定量PCR检测Eos CRSwNP鼻黏膜中let-7a的表达;采用免疫组化方法检测BAFF蛋白质表达水平;应用Spearman's检验分析let-7a与BAFF的相关性.结果 let-7a在Eos CRSwNP表达明显下降(P＜0.05),BAFF蛋白质表达升高(P＜0.05),两者均表达于上皮细胞且呈负相关(r=-0.66,P＜0.05).结论 let-7a可能通过调控BAFF,参与EosCRSwNP中嗜酸粒细胞的活化.
목적 초보탐토기산립세포형적반유비식육적만성비-비두염(Eos CRSwNP)발병과정중miRNA대B림파세포자격인자(BAFF)적조공영향.방법 수집2012년1월지2013년2월재본원치료적Eos CRSwNP환자비점막표본10례화정상비점막표본10례.운용생물신식학연건(Pictar、TargetScan급miRanda)예측대BAFF진행조공적miRNA.운용실시정량PCR검측Eos CRSwNP비점막중let-7a적표체;채용면역조화방법검측BAFF단백질표체수평;응용Spearman's검험분석let-7a여BAFF적상관성.결과 let-7a재Eos CRSwNP표체명현하강(P＜0.05),BAFF단백질표체승고(P＜0.05),량자균표체우상피세포차정부상관(r=-0.66,P＜0.05).결론 let-7a가능통과조공BAFF,삼여EosCRSwNP중기산립세포적활화.
Objective To explore the regulatory effect of miRNA on B-cell activating factor (BAFF) in chronic eosinophilic rhinosinusitis with nasal polyp (Eso CRSwNP).Methods Ten nasal mucosal samples were collected from Eso CRSwNP patients who were admitted and treated in our hospital between January 2012 and February 2013.Normal nasal mucosal tissues (n =10) served as control.The miRNA-targeting BAFF was predicted by bioinformatics tools.Immunohistochemistry and real time polymerase chain reaction (RT-PCR) were used to assess the protein expression of BAFF and the predicted miR-NA.The correlation between the predicted miRNA and BAFF was analyzed.Results Down-regulated expression of let-a was confirmed in Eos CRSwNP,while the BAFF protein expression was increased.Let-a was positively correlated with BAFF in nasal epithelia.Conclusions Let-a might contribute to mucosal eosinophilia in eosinophilic CRSwNP via targeting BAFF.