CAJ | 학술논문

目的观察右美托咪定对脓毒症大鼠不同时期炎症介质的作用及其对生存率的影响.方法将90只雄性Wistar大鼠随机(随机数字法)分为三组:正常对照组(C组),脓毒症模型组(S组),右美托咪定治疗组(D组).正常对照组静脉注射等量的生理盐水,其余两组通过静注内毒素构建脓毒症模型,并给予相应不同的处理,按时间点取血,观察造模48 h内各组大鼠的行为改变和病死率;应用酶联免疫吸附法检测血液中的早期炎症介质肿瘤坏死因子-α、白介素-6和晚期炎症介质高迁移率族蛋白B1的含量变化.结果三组间大鼠病死率存在显著差别,肿瘤坏死因子α、白介素6、高迁移率族蛋白B1血清水平存在显著差异.结论右美托咪定对脓毒症大鼠早期和晚期炎症介质均有明显的抗炎作用,并可降低脓毒症大鼠病死率.
목적 관찰우미탁미정대농독증대서불동시기염증개질적작용급기대생존솔적영향.방법 장90지웅성Wistar대서수궤(수궤수자법)분위삼조:정상대조조(C조),농독증모형조(S조),우미탁미정치료조(D조).정상대조조정맥주사등량적생리염수,기여량조통과정주내독소구건농독증모형,병급여상응불동적처리,안시간점취혈,관찰조모48 h내각조대서적행위개변화병사솔;응용매련면역흡부법검측혈액중적조기염증개질종류배사인자-α、백개소-6화만기염증개질고천이솔족단백B1적함량변화.결과 삼조간대서병사솔존재현저차별,종류배사인자α、백개소6、고천이솔족단백B1혈청수평존재현저차이.결론 우미탁미정대농독증대서조기화만기염증개질균유명현적항염작용,병가강저농독증대서병사솔.
Objective To investigate the effect of dexmedetomidine (DEX) on inflammatory mediators in different periods of sepsis and its impact on survival rate of rats with sepsis.Methods Ninety male Wistar rats were randomly divided into three groups:control group (group C),sepsis model group (group S),and DEX treatment group (group D).Lipopolysaccharide (LPS) was injected to the vein to reproduce a classic sepsis model,while in control group,equivalent volume of NS was used instead.Different treatments were given to the corresponding groups,and then venous blood was taken at given intervals.Behavioral changes and mortality rate of rats within 48 hours after experiment were recorded.The levels of the early inflammatory mediators such as tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6) and the late inflammatory mediators such as high-mobility group boxl protein (HMGB1) in blood serum were detected by ELISA.Results Among three groups,there were significant differences in mortality rate of rats,the serum level of TNF-αt,IL-6 and HMGB1 (all P ＜ 0.05).Conclusion DEX has a certain anti-inflammatory effects on early and late stages of sepsis in rats with sepsis,and can also reduce the mortality rate.