CAJ | 학술논문

目的分析非典型布鲁杆菌的16S rDNA序列,评价16S rDNA序列测定方法鉴定布鲁杆菌的可行性.方法用常规方法对l株非典型菌株进行初步鉴定;提取菌株的DNA,双向测定16S rDNA全序列,在NCBI对该序列进行Blast比对,用DNAMAN软件进行菌株的16S rDNA序列一致性比对;同时,从GenBank下载与布鲁杆菌有血清学交叉反应菌株的16S rDNA全序列,用MEGA 6.0构建16S rDNA系统发生树.结果常规鉴定显示,试验菌株为非典型布鲁杆菌.试验菌株的16S rDNA序列与布鲁杆菌基因的相似性为99％,与牛种布鲁杆菌544A、羊种布鲁杆菌16M的16S rDNA序列的一致性为96.99％.系统发生树显示,试验菌株为布鲁杆菌,且与牛种布鲁杆菌544A、羊种布鲁杆菌16M有较为密切的亲缘关系.结论试验菌株为非典型布鲁杆菌,16S rDNA序列测定是一种简便、快速、准确的非典型布鲁杆菌鉴定方法.
목적 분석비전형포로간균적16S rDNA서렬,평개16S rDNA서렬측정방법감정포로간균적가행성.방법 용상규방법대l주비전형균주진행초보감정;제취균주적DNA,쌍향측정16S rDNA전서렬,재NCBI대해서렬진행Blast비대,용DNAMAN연건진행균주적16S rDNA서렬일치성비대;동시,종GenBank하재여포로간균유혈청학교차반응균주적16S rDNA전서렬,용MEGA 6.0구건16S rDNA계통발생수.결과 상규감정현시,시험균주위비전형포로간균.시험균주적16S rDNA서렬여포로간균기인적상사성위99％,여우충포로간균544A、양충포로간균16M적16S rDNA서렬적일치성위96.99％.계통발생수현시,시험균주위포로간균,차여우충포로간균544A、양충포로간균16M유교위밀절적친연관계.결론 시험균주위비전형포로간균,16S rDNA서렬측정시일충간편、쾌속、준학적비전형포로간균감정방법.
Objective To sequence an atypical Brucella strain 16S rDNA,and to evaluate the feasibility of 16S rDNA sequencing method for identification of Brucella.Methods Preliminary identification of atypical strains was carried out with conventional method.Strain DNA was extracted,and 16S rDNA complete sequence was bidirectional sequenced,and Blast in NCBI and DNAMAN software were used for comparison of the sequence identities of the 16S rDNA.Moreover,16S rDNA complete sequence of the stains those were known to cross-react serologically with Brucella was downloaded from GenBank,MEGA 6.0 was used to construct the phylogenetic tree.Results The conventional identification results revealed that it was an atypical Brucella,the gene similarity between the sequences of the test strain 16S rDNA and Brucella was 99％,between 16S rDNA sequence of Brucella abortus 544A and Brucella melitensis 16M was 96.99％.Phylogenetic tree revealed that the test stain was a Brucella,and closely related to Brucella abortus 544A and Brucella melitensis 16M.Conclusion The test strain is an atypical Brucella,and 16S rDNA sequencing analysis is a simple,rapid,and accurate identification method for atypical Brucella.