CAJ | 학술논문

目的了解新疆阿拉山口艾比湖湿地主要蜱种莱姆病螺旋体感染与基因型状况.方法 2014年4-8月,在新疆艾比湖湿地选取3个采集点,采用布旗法采集游离蜱,经形态学鉴定蜱种后,采用巢式PCR法扩增莱姆病病原5S-23S rRNA基因,将PCR产物测序并进行BLAST比对分析.结果共检测游离蜱152只,其中血红扇头蜱12只、亚洲璃眼蜱59只、边缘革蜱81只.各蜱种莱姆病病原总体阳性率为34.87％(53/152),其中亚洲璃眼蜱阳性率为(57.63％,34/59),边缘革蜱阳性率为(22.22％,18/81),血红扇头蜱阳性率为(8.33％,1/12),亚洲璃眼蜱的阳性率高于边缘革蜱和血红扇头蜱(x2=18.328、9.694,P均＜0.05);5S-23SrRNA基因测序比对显示艾比湖湿地莱姆病基因型为伯氏疏螺旋体.结论首次在新疆阿拉山口艾比湖湿地硬蜱检测到伯氏疏螺旋体,提示新疆艾比湖为莱姆病病原自然疫源地.
목적 료해신강아랍산구애비호습지주요비충래모병라선체감염여기인형상황.방법 2014년4-8월,재신강애비호습지선취3개채집점,채용포기법채집유리비,경형태학감정비충후,채용소식PCR법확증래모병병원5S-23S rRNA기인,장PCR산물측서병진행BLAST비대분석.결과 공검측유리비152지,기중혈홍선두비12지、아주리안비59지、변연혁비81지.각비충래모병병원총체양성솔위34.87％(53/152),기중아주리안비양성솔위(57.63％,34/59),변연혁비양성솔위(22.22％,18/81),혈홍선두비양성솔위(8.33％,1/12),아주리안비적양성솔고우변연혁비화혈홍선두비(x2=18.328、9.694,P균＜0.05);5S-23SrRNA기인측서비대현시애비호습지래모병기인형위백씨소라선체.결론 수차재신강아랍산구애비호습지경비검측도백씨소라선체,제시신강애비호위래모병병원자연역원지.
Objective In order to understand the major tick species,Borrelia burgdorferi infection and genotype status at Aibi Lake,Alataw Pass,Xinjiang Autonomous Region.Methods Free-living ticks were collected by drag-flag method from April to August,2014,and the morphological identification was carried out.Borrelia spp.was detected by 5S-23S rRNA gene primers.The PCR products were sequenced and analyzed with BLAST.Results One hundred fifty-two isolated ticks were collected,including Rhipicephalus sanguineus (12),Hyalomma asiaticum (59) and Dermacentor Marginatus (81).The positive rate of Borrelia spp.was 34.87％ (53/152).The positive rate of Hyalomma asiaticum (57.63％,34/59) was higher than that of Dermacentor Marginatus (22.22％,18/81,x2 =18.328,P ＜ 0.05) and Rhipicephalus sanguineus (8.33％,1/12,x2 =9.694,P ＜ 0.05).The analysis of 5S-23S rRNA sequencing indicated the pathogen was Borrelia burgdorferi sensu stricto.Conclusions Borrelia burgdorferi sensu stricto in ticks is firstly detected in Alataw region.The results conveyed us Aibi Lake was natural epidemic focus of Lyme disease.