CAJ | 학술논문

对甘草(Glycyrrhiza uralensisFisch)的有效部位进行富集和活性成分含量测定,同时结合4种抗氧化模型比较分析了甘草组培和栽培活性主要成分含量及差异.结果表明:组培甘草细胞中多糖含量(21.6±0.1)%,高于栽培甘草多糖含量(19.2±0.2)%,;黄酮含量两者相当,分别为(30.6±1.9)%,和(31.2±1.5)%,;而组培甘草中皂苷的含量为(7.0± 0.2)%,,显著低于栽培甘草含量(45.4±1.1)%,.采用普鲁士兰法、水杨酸比色法及 DPPH 法检测不同组分抗氧化活性,结果表明组培甘草不同有效部位活性均优于栽培品种;β–胡萝卜褪黑素法表明组培甘草具有一定的抗氧化活性,但弱于栽培甘草.综上所述,从组培甘草细胞中获取甘草多糖和黄酮类成分具有较好的抗氧化能力.
대감초(Glycyrrhiza uralensisFisch)적유효부위진행부집화활성성분함량측정,동시결합4충항양화모형비교분석료감초조배화재배활성주요성분함량급차이.결과표명:조배감초세포중다당함량(21.6±0.1)%,고우재배감초다당함량(19.2±0.2)%,;황동함량량자상당,분별위(30.6±1.9)%,화(31.2±1.5)%,;이조배감초중조감적함량위(7.0± 0.2)%,,현저저우재배감초함량(45.4±1.1)%,.채용보로사란법、수양산비색법급 DPPH 법검측불동조분항양화활성,결과표명조배감초불동유효부위활성균우우재배품충;β–호라복퇴흑소법표명조배감초구유일정적항양화활성,단약우재배감초.종상소술,종조배감초세포중획취감초다당화황동류성분구유교호적항양화능력.
The effective parts of Glycyrrhiza uralensis Fisch were enriched and the active ingredients were detected. Four antioxidization models were used to compare the activity of the cultivated Glycyrrhiza uralensis Fisch with that of thenative one. The result showed the yield of polysaccharides in the former was(21.6±0.1)%, while in the latter,it was(19.2± 0.2)%,. Their flavonoids were similar,(30.6±1.9)% to(31.2±1.5)%,. Whereas,the triterpenes in the cultivated ones was (7.0±0.2)%,significantly lower than that in the native ones,(45.4±1.1)%,. According to the reducing power,salicylic acid method and DPPH radical scavenging assay,the cultivated Glycyrrhiza uralensis Fisch's flavonoids and triterpenes pos-sessed better anti-oxidant activity than the native ones. But based on the bleaching ability ofβ-carotene in linoleic acid system,the native Glycyrrhiza uralensis Fisch's flavonoids and triterpenes possessed a better anti-oxidant activity. In conclu-sion,there's a considerable amount of polysaccharides and flavonoids in the cultivated Glycyrrhizauralensis Fisch which can be used as a potential agent for antioxidization.